Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl

Refine search results

Journals help

  1. 1 Open Chemistry

Years help

  1. 1 2006

Authors help

  1. 1 He Z.

  2. 1 Jia C.

  3. 1 Qi W.

  4. 1 Qiao B.

  5. 1 Yang H.

Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 1

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  scavenger
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1
Content available remote

Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry

100%
Jia C., Qi W., He Z., Yang H., Qiao B.
Open Chemistry
|
2006
|
vol. 4
|
issue 2
285-298
EN
Two heptapeptides have been prepared by Fmoc methodology using Wang resin as solid support. For attachment of the first amino acid, several coupling systems were evaluated, and DIC/DMAP system could give yields of >99% and low levels of racemization. The selection of scavenger combination to deprotect side chains revealed that H2O/p-cresol was good at scavenging trityl and 1,2-ethanedithiol was highly efficient for scavenging t-butyl. Through shortening the preactivation time to 5 min, the racemization which occurred during formation of amide bonds coupled by HBTU was minimized. The crude peptides were characterized by RP-HPLC and MS, and sequenced by MS/MS to acquire reliable amino acid sequence information.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.