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Mitochondria-associated satellite I RNA binds to hnRNP K protein

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Klimek-Tomczak K., Mikula M., Dzwonek A., Paziewska A., Wyrwicz L. S., Hennig E. E., Ostrowski J.

2006

vol. 53

issue 1

169-178

hnRNP K protein, which localizes to the nucleus, cytoplasm and mitochondria, is involved in the various cellular processes that compose gene expression. We used a SAGE-based assay to profile RNAs associated with hnRNP K protein in rat mitochondria. RNA was isolated from mitoplasts obtained from highly purified and RNase-treated mitochondria. Total RNA and RNA associated with hnRNP K protein were then used as input material for generating two SAGE libraries. Mitochondrion-derived tags isolated from the total mitoplast RNA library represented 86.3%, while those isolated from the library constructed from RNA associated with hnRNP K protein represented only 28.2% of selected tags. Thus, an unexpected number of nuclear-encoded RNAs were purified from mitochondria. Many of these transcripts were co-purified with hnRNP K protein, and high levels of nuclear-encoded RNAs co-immunoprecipitating with K protein corresponded to elevated hnRNP K protein levels of the organelle. The most abundant RNAs that were co-purified with hnRNP K protein represented transcripts originating from satellite I DNA. While satellite I RNA levels were higher in the nucleus and cytoplasm than in mitochondria, the most abundant binding of satellite I transcripts to hnRNP K protein was found in mitochondria. The role of satellite I RNA in mitochondria remains to be elucidated.

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1 Acta Biochimica Polonica

1 2006

1 Dzwonek A.

1 Hennig E. E.

1 Klimek-Tomczak K.

1 Mikula M.

1 Ostrowski J.

1 Paziewska A.

1 Wyrwicz L. S.

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Mitochondria-associated satellite I RNA binds to hnRNP K protein