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Global quantification of heterochromatin-associated histone methylations in cell lines with differential sensitivity to ionizing radiation

100%

Cetinkaya M., Özgür E., Dalay N., Gezer U.

2015

vol. 62

issue 2

173-176

Histone modifications are involved in the DNA damage response (DDR). Here, by utilizing an ELISA immunoassay we assessed the methylation at H3K9 (H3K9me2 and H3K9me3) in two cell lines with differential sensitivity to radiation-induced apoptosis, HeLa (sensitive) and MCF-7 (resistant). We found that DNA damage induction by γ-irradiation leads to considerable accumulation (up to 5-fold) of H3K9me2 and H3K9me3, but not of H4K20me3 (control modification) in MCF-7 cells (p<0.05). Interestingly, a lower dose (2 Gy) was more effective than 5 Gy. In HeLa cells a smaller effect (approx. 1.5-1.8-fold) was evident only at 5 Gy. In conclusion, our findings reveal that DNA damage leads to specific accumulation of H3K9me2 and H3K9me3 in a cell-type specific manner.

Squamous cell oesophageal cancer in retinoblastoma survivor: does germinal RB1 mutation influence chemo- and radiosensitivity?

75%

Dworzecki T., Smolska-Ciszewska B., Suwiński R.

OncoReview

2014

vol. 4

issue 2

A72-75

The case of complete response of squamous cell oesophageal cancer to radiochemotherapy in patient previously operated due to hereditary retinoblastoma is presented. Second cancers in retinoblastoma survivors are the main cause of death in that group of patients in developed countries. There is little data on the outcome of treatment in those patients but the few papers available suggest rather poor prognosis. However, it is not clear whether the biology of cancer determined by RB gene mutation is responsible for that. The data suggesting decreased radio- and chemoresistance in cancers with decreased RB gene expression is discussed in the present paper. The role of that gene in the response to cancer treatment was showed in lung, breast and bladder cancer. Moreover, laboratory studies seem to confirm clinical observations and show that RB gene inhibition leads to increased cytotoxic effect of cisplatin, etoposide and 5-fluorouracil. It seems that without the incorporation of gene expression profiling into clinical practice further improvement in cancer treatment will be difficult to achieve.

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1 Acta Biochimica Polonica

1 OncoReview

1 2015

1 2014

1 Cetinkaya M.

1 Dalay N.

1 Dworzecki T.

1 Gezer U.

1 Smolska-Ciszewska B.

1 Suwiński R.

1 Özgür E.

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Global quantification of heterochromatin-associated histone methylations in cell lines with differential sensitivity to ionizing radiation

Squamous cell oesophageal cancer in retinoblastoma survivor: does germinal RB1 mutation influence chemo- and radiosensitivity?