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1

DNA vs RNA based studies of nitrogen removal bacteria genes via qPCR

100%

Banach-Wiśniewska A., Gamoń F., Ziembińska-Buczyńska A., Banach-Wiśniewska A., Gamoń F., Ziembińska-Buczyńska A.

Archives of Environmental Protection

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2021

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vol. 47

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issue 1

2

Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori

100%

Pan Y., Xia H., Lü P., Chen K., Yao Q., Chen H., Gao L., He Y., Wang L.

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issue 4

671-677

EN

Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.

3

Feeding state and age dependent changes in melanin-concentrating hormone expression in the hypothalamus of broiler chickens

86%

Simon Á., Németh J., Jávor A., Komlósi I., Bai P., Oláh J., Juhász B., Kiss R., Szilvássy Z., Czeglédi L.

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2018

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vol. 65

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issue 2

251-258

EN

We aimed to quantify the gene expression changes of the potent orexigenic melanin-concentrating hormone (MCH) in chicken (Gallus gallus) hypothalamus with quantitative real-time polymerase chain reaction (qPCR), and for the first time determine peptide concentrations with a novel radioimmunoassay (RIA) under different feeding status. Three different experimental conditions, namely ad libitum feeding; fasting for 24 h; fasting for 24 h and then refeeding for 2 h, were applied to study changes of the aforementioned target and its receptor (MCHR4) gene expression under different nutritional status. The relative changes of MCH and MCHR4 were also studied from 7 to 35 days of age. Expression of PMCH and MCHR4 along the gastrointestinal tract (GIT) was also investigated. We found that expression of both targets was significant in the hypothalamus, while only weak expression was detected along the GIT. Different nutritional states did not affect the PMCH and MCHR4 mRNA levels. However, fasting for 24 h had significantly increased the MCH-like immunoreactivity by 25.65%. Fasting for 24 h and then refeeding for 2 h had further significantly increased the MCH peptide concentration by 32.51%, as compared to the ad libitum state. A decreasing trend with age was observable for both, the PMCH and MCHR4 mRNA levels, and also for the MCH-like immunoreactivity. Correlation analysis did not result in a significant correlation between MCH peptide concentration and abdominal fat mass in ad libitum fed birds. In conclusion, MCH peptide concentration altered in response to 24 h fasting, which indicated that this peptide may take part in feed intake regulation of broiler chickens.

4

Soil microbiomes of reclaimed and abandoned mines of the Yamal region

72%

Pershina E., Ivanova E., Kimeklis A., Zverev A., Kichko A., Aksenova T., Andronov E., Abakumov E., Pershina E., Ivanova E., Kimeklis A., Zverev A., Kichko A., Aksenova T., Andronov E., Abakumov E.

Polish Polar Research

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2020

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vol. 41

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issue 1

Refine search results

DNA vs RNA based studies of nitrogen removal bacteria genes via qPCR

Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori

Feeding state and age dependent changes in melanin-concentrating hormone expression in the hypothalamus of broiler chickens

Soil microbiomes of reclaimed and abandoned mines of the Yamal region