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100%
EN
The plant Solanum nigrum treated with the pathogen Phytophthora infestans-derived elicitor responded by elevated reactive oxygen species (ROS) production, lipid peroxidation and lipoxygenase (EC 1.13.11.12) activity in comparison with control plants indicating that oxidative stress took place. We demonstrate that these events are accompanied by a significant increase in plastoquinone (PQ) level. It is postulated that PQ may be associated with mechanisms maintaining a tightly controlled balance between the accumulation of ROS and antioxidant activity that determines the full expression of effective defence.
EN
The study surveyed plants used in ethno-veterinary practice among the Fulani people of Girei, Adamawa State, Nigeria. Herein, a total of 100 key guided respondents were interviewed or took part in focus group discussion, mainly Jauro, Jemila and Ardo herdsmen along the Girei-Mubi road who were highly experienced and inherited ethno-veterinary knowledge from their fore-fathers. The results identified 30 medicinal plants species and the different livestock diseases conditions they treat. The plant names in Fulani, the parts of plants used and mode of preparations were also documented. In the study, we also noted the significant employment of cow milk (Madara or Nunu) and evaporites or potash (Kanwa) as recipes in livestock diseases management. Of the identified plants, the leaves (61.29%) were commonly used, then bark (25.80%), root (6.45%) and fruits (6.45%). The phytochemical screening of the available plants showed the presence of secondary metabolites such as saponins, alkaloids, tannins, anthraquinone and steroids/triterpenes. These are thought responsible for the antibacterial, antifungal, wound healing, anti-venom, and other pharmacological activities. As a result of our work, we concluded that the identified plant material, when extracted, purified and isolated could be used in the development of new drugs for livestock management.
EN
Bioactive polyphenolics are ubiquitously present in plants and may play an important role in the prevention and management of certain human diseases. Three known flavonoids viz Kaemperol-3-O-rutinoside (1), quercetin-3-O-glucoside (2) and kaemperol-3-O-glucoside (3) and inseparable mixture (1:1) of quercetin-3-O-glucose/galactose (4) were isolated, and identified for the first time from Holarrhena floribunda. The antioxidant capacity using the ORAC, FRAP and TEAC assays and inhibition of lipid peroxidation were measured for isolated flavonoids. The result showed that compounds 2 and 4 showed significantly increased ORAC, TEAC, and FRAP activities with low pro-oxidant potential as well as improved lipid peroxidation inhibition levels when compared to compounds 1 and 3. The most active compounds were found to be flavonoids with a quercetin basic structure. These results imply that the isolated flavonoid glycosides are responsible for the antioxidant activity of the plant leaves and it forms the scientific basis for its traditional usage.
EN
The concentration of heavy metals in fields used as urban waste dump sites in Owerri, Imo state Nigeria was evaluated. Three dumpsites were selected from Avu (along Port Harcourt Road), Mechanic village (opposite Tipper Park) and Egbeada housing (along Onitsha Road). Site selection was based on the presence of the dumps for at least 10 years. Soil samples were collected from the dumpsites and 100 m away from the dumpsite as control, using soil auger at depth of 0 ‒15 cm and 15‒30 cm. Cassava crops were planted on all the sites and the cassava roots were sampled alongside the soil for the study of heavy metal contents in plant roots. Routine soil analysis and plant root analysis were carried out to determine the physio-chemical characteristics and heavy metal contents of the soil and plant roots. Results showed that heavy metal contents of soils and plants are significantly (p = 0.05) higher in the dumpsites than the control except for Cd which did not show significant difference. Also results showed that Cd and Pb content of soil and plants are higher (4.17 and 3.17 mg/kg, respectively) for soil and (0.2 and 0.1 mg/kg, respectively) for plant roots. It is therefore recommended that farmers should avoid growing crops in urban waste dumpsites to avoid food contamination and health hazard.
7
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Plant ureases: Roles and regulation.

88%
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2000
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vol. 47
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issue 4
1189-1195
EN
Both urea and urease were subjects of early scientific investigations. Urea was the first organic molecule to be synthesized and jack bean urease was the first enzyme ever to be crystallized. About 50 years later it was shown to be the first nickel metalloenzyme. Since then, nickel-dependent ureases have been isolated from many bacteria, fungi and higher plants. They have similar structures and mechanisms of catalysis. A urease apoenzyme needs to be activated. This process requires participation of several accessory proteins that incorporate nickel into the urease forming catalytic site. In this review, ureases from various organisms are briefly described and the similarities of their structures discussed. Moreover, the significance of urea recycling in plants is explained and recent literature data about the function and activation of plant ureases are presented.
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2017
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vol. 64
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issue 3
407-413
EN
Bisphenol A (BPA) is a widely used chemical, that can potentially be toxic to plants. In this study we examined the toxicity of 5-50 mg/l of BPA on Arabidopsis thaliana. Additionally, the effects of 0.5-5 mg/l of BPA were examined after four weeks of development. BPA had no effect on the germination rate and the chlorophyll a/b ratio. The chlorophyll a and carotenoid content was significantly elevated in seedlings treated with 5 mg/l of BPA. In 4-week-old plants there was no change in the chlorophyll and carotenoid content and photosynthetic parameters (Fv/Fm, Fv/F0 and PI) were unaffected, which suggests no photoinhibition. No oxidative stress symptoms were observed. BPA significantly decreased leaf protein content. A low concentration of BPA seems to have no significant effect on A. thaliana flowering, but further investigation is needed. The results obtained indicate that a low concentration of BPA has no negative effect on the growth and development of A. thaliana.
EN
The main objective of this research is to determine the content of metals (Ca, Cu, Fe, Mg and Zn) and total phenols in different plant extracts of Moringa oleifera, Cassia tora, Ocimum gratissimum, Vernonia baldwinii and Telfairia occidentalis. Content were determined using Atomic Absorption Spectroscopy. The result indicate that Moringa oleifera plant extracts range from 0.25 ±0.00 to 6.13 ±0.30 mg/kg, Cassia tora plant extracts - 0.17 ±0.03 to 7.48 ±0.06 mg/kg, Ocimum gratissimum plant extracts - 0.18 ±0.00 to 5.43 ±0.12 mg/kg, Vernonia baldwinii and Telfairia occidentalis plant extracts - 0.21 ±0.03 to 7.86 ±0.12 mg/kg and 0.17 ±0.00 to 4.52 ±0.06 mg/kg, respectively. The results also revealed a lower abundance of heavy metals. The total phenolic content was determined using the modified Folin-Ciocalteu method. Herein, the phenolic content in Moringa oleifera was 8.50 ±1.23 mg Garlic Acid Equivalent g-1 (mg GAE g-1), Cassia tora - 30.00 ±0.00 mg GAE g-1, Ocimum gratissimum - 45.00 ±1.41 mg GAE g-1 , Vernonia baldwinii - 49.00 ±1.14 mg GAE g-1 and Telfairia occidentalis - 46.6 7 ±0.27 mg GAE g-1. We found the lowest total phenol content in Moringa oleifera. This also possessed high chelating activity. In contrast, Vernonia baldwinii contained the highest total phenol content, but had low chelating activity. The plant extracts with high levels of phenolic compounds exhibited good antioxidant activity.
EN
BACKGROUND: Trapa natans is native to Euroasia, Africa and is naturalized in North America. Trapa natans is an aquatic plants that lives in small, fertile natural reservoirs sheltered from the wind. In Poland Water Chestnut is rare and protected species, which acquired CR (Critically Endangered) category in Polish Red Data Book of Plants. In Europe it is protected by virtue of the Bern Convention. MATERIAL AND METHODS: The fi eld research was carried in 2007-2008 using the standard fl oristic investigation method. RESULTS/CONCLUSIONS: The occurrence of Water Chestnut in Kuźni Raciborskiej was fi rst reported by Schube, subsequently confi rmed by Piórecki and Urbisz. In 2007-2008 the locality was reconfi rmed during the research conducted by this area. Currently, the population of species is very abundant.
PL
WSTĘP: Trapa natans naturalnie występuje w Euroazji, Afryce i jest gatunkiem naturalizowanym w Ameryce Północnej. Kotewka orzech wodny to wodna roślina, żyjąca w małych, urodzajnych naturalnych zbiornikach chronionych od wiatru. W Polsce jest rzadkim i chronionym gatunkiem, który uzyskał kategorię CR (krytycznie zagrożony wyginięciem). W Europie jest objęta ochronną na podstawie Konwencji Berneńskiej. MATERIAŁ I METODY: W latach 2007-2008 prowadzono badania terenowe standardową metodą fl orystyczną. WYNIKI I WNIOSKI: Występowanie Trapa natans w Kuźni Raciborskiej po raz pierwszy zostało odnotowane przez Schube, później potwierdzone przez Pióreckiego i Urbisza. Podczas badań terenowych prowadzonych w latach 2007-2008 występowanie kotewki w Kuźni Nieborowskiej zostało potwierdzone. Obecnie populacja gatunku jest bardzo obfita.
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