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EN
Excess boron in soil is often found in dry agricultural areas and is one of the factors limiting crop yields. Polysaccharide-based biostimulants can mitigate the harmful effects of environmental stresses on plants. I performed this study under controlled greenhouse conditions to understand the response of pepper plants under boron stress to treatment with water-soluble chitosan (WSC). I watered plants with 50 or 100 mg/l WSC and 1.5 mM boric acid solution. As a result of WSC application at both concentrations, plants were taller and had more leaves and greater leaf length, width, relative chlorophyll content, and fresh aboveground weight compared with the control. Plants exposed to boron stress had fewer leaves, a lower relative chlorophyll content, and leaf blade damage indicative of boron toxicity. At the same time, boron-exposed plants showed a marked increase in the leaf nitrogen, phosphorus, potassium, and boron contents. Applying WSC at both concentrations modulated boron stress in plants by improving plant growth; reducing boron accumulation in leaves; and increasing the available nitrate nitrogen, phosphorus, and potassium contents in the substrate.
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2000
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vol. 47
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issue 2
427-434
EN
Expression as well as properties of integrins are altered upon transformation. Cell adhesion regulated by integrins is modulated by glycosylation, one of the most frequent biochemical alteration associated with tumorogenesis. Characterisation of carbohydrate moieties of α3β1 integrin on the cultured human bladder carcinoma (T-24, Hu456, HCV 29T) and human normal ureter and bladder epithelium (HCV 29, Hu609) cell lines was carried out after an electrophoresis and blotting, followed by immunochemical identification of α3 and β1 integrin chains and analysis of their carbohydrates moieties using highly specific digoxigenin-labelled lectins. In all the studied cell lines α3β1 integrin was glycosylated although in general each subunit differently. Basic structures recognized in β1 subunit were tri- or tetraantennary complex type glycans in some cases sialylated (T-24, HCV 29, HCV 29T) and fucosylated (Hu609, HCV 29T). Positive reaction with Phaseolus vulgaris agglutinin and Datura stramonium agglutinin suggesting the presence of β1-6 branched N-linked oligosaccharides was found in cancerous cell lines (T-24, Hu456) as well as in normal bladder epithelium cells (Hu609). High mannose type glycan was found only in β1 subunit from Hu456 transitional cell cancer line. On the other hand α3 subunit was much less glycosylated except the invasive cancer cell line T-24 where high mannose as well as sialylated tri- or tetraantennary complex type glycans were detected. This observation suggests that changes in glycosylation profile attributed to invasive phenotype are rather associated with α3 not β1 subunit.
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