Search results

1

The role of microtubules in electrotaxis of rat Walker carcinosarcoma WC256 cells

100%

Krecioch I., Madeja Z., Lasota S., Zimolag E., Sroka J.

|

2015

|

vol. 62

|

issue 3

401-406

EN

The endogenous electric field may provide an important signal for directional cell migration during cancer metastasis but the mechanism of cell electrotaxis is poorly understood. It was postulated that microtubules play a central role in the polarization and directional migration of several types of cells. In this paper we investigated the role of microtubules in electrotaxis of rat Walker carcinosarcoma WC256 cells. We found that colchicine-stimulated disassembly of microtubules caused the formation of blebs instead of lamellipodia at the front of about 45% of cells. Most of the remaining cells contracted and became rounded or transformed into non-polar cells. Depolymerization of microtubules in both subpopulations of cells reduced the directionality of cell migration to about 50% of the control, but bleb- forming cells migrated much more efficiently than lamellipodia-forming cells. The analysis of microtubules architecture in the presence of an endogenous electric field showed that there is no relationship between the direction of migration and the polarization of microtubules. These results suggest that microtubules are not indispensable for electrotaxis of WC256 cells, however they may improve the directionality of cell migration.

2

The role of actin and microtubule networks in plasmid DNA intracellular trafficking

100%

Ondřej V., Lukášová E., Falk M., Kozubek S.

|

2007

|

vol. 54

|

issue 3

657-663

EN

This work is focused on the function of the microtubule and actin networks in plasmid DNA transport during liposomal transfection. We observed strong binding of plasmid DNA-lipid complexes (lipoplexes) to both networks and directional long-range motion of these lipoplexes along the microtubules. Disruption of either of these networks led to the cessation of plasmid transport to the nucleus, a decreased mobility of plasmids, and accumulation of plasmid DNA in large aggregates at the cell periphery. Our findings show an indispensable but different role of both types of cytoskeleton, actin and microtubular, in the processes of gene delivery.

3

Białka z rodziny +tip - "agenci" śledzący mikrotubule w rozwoju i plastyczności neuronów

63%

Jaworski J.

Kosmos

|

2018

|

vol. 67

|

issue 1

139-149

PL

Mikrotubule są jednym z elementów cytoszkieletu a ich rolą jest zapewnienie prawidłowego transportu wewnątrzkomórkowego, utrzymanie kształtu komórek oraz generowanie sił mechanicznych. Aby mikrotubule mogły pełnić swoje funkcje komórkowe konieczna jest liczna grupa białek je wiążących, odpowiedzialnych za ich polimeryzację, stabilizację i dynamikę. Należą do nich między innymi białka śledzące koniec plus mikrotubul (ang. microtubule plus-end tracking proteins, +TIP). W ciągu ostatnich 10 lat poczyniono ogromne postępy w rozumieniu zarówno podstawowych aspektów działania tych białek na poziomie molekularnym, jak i ich udziału w rozwoju i plastyczności komórek nerwowych. Celami niniejszego artykułu są: zapoznanie czytelnika z podstawowymi informacjami na temat białek +TIP oraz z rolą jaką pełnią one w neuronach w trakcie powstawania aksonu, dendrytów i plastyczności synaptycznej.

EN

The role of microtubules, one of the three components of cytoskeleton, is to ensure proper intracellular transport, maintain cell shape and generate mechanical forces. In order to fulfill by microtubules their cellular functions, a large number of binding proteins responsible for their polymerization, stabilization or dynamics are needed. These include inter alia plus-end tracking proteins (+TIPs). Over the past 10 years, a great progress has been made in terms of understanding both, the fundamental aspects of these molecules at molecular level and their contribution to the development and plasticity of nerve cells. The purpose of this article is to provide the readers the basic information about the +TIP proteins and the role they play in neurons in the formation of axon, dendrites and synaptic plasticity.

4

Oddziaływanie białka prionowego z mikrotubulami

63%

Nieznańska H.

Kosmos

|

2018

|

vol. 67

|

issue 1

121-130

PL

Misfolded prion protein (PrP ) is known as a major agent leading to infectious neurodegenerative diseases, known as transmissible spongiform encephalopathies (TSE). The mechanism of conversion of the physiological form of prion protein (PrP C ) into the pathological PrP TSE as well as the identity of neurotoxic form of this protein is not fully characterized. Under physiological conditions, PrP C one, is predominantly extracellular, tethered to the plasma membrane surface through the GPI anchor. However, cytosolic forms of PrP, termed as cytoPrP have also been found. Interestingly, a significant increase in the concentration of cytoPrP is observed in TSE. Recently, it was shown that mislocalized PrP can be a neurotoxic agent. The mechanism of neurotoxicity might be linked to the direct interaction of this form of PrP with tubulin. This interaction leads to tubulin aggregation, inhibition of microtubules (MT) assembly, disruption of microtubular cytoskeleton and eventually cell death. MT stabilization, by decreasing the level of MAP phosphorylation, can protect neurons from toxic effect of cytosolic forms of PrP.

Refine search results

2 Acta Biochimica Polonica

2 Kosmos

2 2018

1 2015

1 2007

1 Falk M.

1 Jaworski J.

1 Kozubek S.

1 Krecioch I.

1 Lasota S.

1 Lukášová E.

1 Madeja Z.

1 Nieznańska H.

1 Ondřej V.

1 Sroka J.

1 Zimolag E.

The role of microtubules in electrotaxis of rat Walker carcinosarcoma WC256 cells

The role of actin and microtubule networks in plasmid DNA intracellular trafficking

Białka z rodziny +tip - "agenci" śledzący mikrotubule w rozwoju i plastyczności neuronów

Oddziaływanie białka prionowego z mikrotubulami