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SENSITIVITY TO CHITOSAN ASCORBATE MICROAEROPHILIC BACTERIA ISOLATED FROM INFECTIONS OF ORAL CAVITY

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Kochańska B., Kędzia A., Gębska A.

Progress on Chemistry and Application of Chitin and its Derivatives

2016

vol. 21

109-113

The aim of the date was to determine the sensitivity to chitosan ascorbate microaerophilic bacteria isolated from pathological pockets, root canals and apical abscesses. Chitosan was obtained from Antarctic krill. The experiments included 70 clinical isolates and 4 standards strains. The susceptibility of microaerophilic bacteria was performed by means of plate dilution technique in Brucella agar supplemented with 5% defibrinated sheep blood. Inoculum contained 105 CFU per spot. Incubation inoculated agar was performed at 37°C for 48 hrs in microaerophilic conditions. MIC was defined as the least concentrations of chitosan ascorbate that inhibited growth of tested bacteria. The results indicated that 47% of all date bacteria was susceptible to chitosan ascorbate in ranges ≤ 0.01 – 0.5 mg/ml. The most susceptible were the strains of Corynebacterium matruchotii (100%, MIC ≤ 0.01 – 0.5 mg/ml) and the least sensitive strains of Rothia dentocariosa (MIC in ranges 1.0 ˗ ≥ 4.0 mg/ml ).

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1 Progress on Chemistry and Application of Chitin and its Derivatives

1 2016

1 Gębska A.

1 Kochańska B.

1 Kędzia A.

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SENSITIVITY TO CHITOSAN ASCORBATE MICROAEROPHILIC BACTERIA ISOLATED FROM INFECTIONS OF ORAL CAVITY