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This research work assessed the antibacterial properties of probiotic bacterial which was isolated from breast milk. The breast milk has a distinct amalgamation of minerals, proteins, lipids, carbohydrates and various vitamins that endorse the proper development, growth and immunity of the children. That’s the reason behind it’s consideration to be a comprehensive and inclusive food for new born babies. Furthermore, it is also abundant in various bioactive compounds which encourage the maturation of the immune system over and above develop body’s defense against infections. This research used a standard methodology to isolate the bacterial. In the midst of these bioactive agents, probiotic bacteria were properly isolated from human milk in this research work by means of selective MRS media. Five Lactobacillus spp. were isolated from every one of the three breast milk samples and two Enterococcus species, were observed as potential probiotics, and identified using morphological and biochemical tests which includes L. rhamnosus, L. plantarum, L. casei, L. fermentum, L. acidophilus, En. faecalis and En. faecium. The isolated bacteria were facultative anaerobic, catalase negative, gram positive and non-endospore forming. Sugar fermentation arrangements of equally isolated bacteria also significantly different. The adding up of breast milk probiotics to children formulas possibly will be an innovative substitute to mimic some of the purposeful consequences of human milk in children who are not breastfed.
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