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Mucuna pruriens-seed extract stimulating immune system in Litopenaeus vannamei against Vibrio harveyi

100%
Shanmugavel G., Krishnamoorthy G.
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vol. 39
95-112
EN
Objective: To evaluate the immunostimulant (ISt) role of Mucuna pruriens-seed (Mp-seed) enriched with Artemia (Ar) in Litopenaeus vannamei (L. vannamei) infected by Vibrio harveyi (V. harveyi). Method: Fifteen shrimp (30±5g) were stocked per 250 L tank in 3 replicates and fed twice daily with Ar enriched with Mp-seed methanolic extracts at 100 ppm and 200 ppm for 21 days. Infected L. vannamei shows altered immunoparameter (IPM) and antioxidant enzyme status due to the V. harveyi infection induced stress. Results: Significantly increased (p<0.05) total haemocyte count, respiratory burst, phenoloxidase and superoxide dismutase activities were observed in shrimp fed with Mp enriched Ar diets compared to infected group. Supplementation of Mp enriched Ar in diet could enhance the immunity of shrimp against V. harveyi infection by reverses the antioxidant level. The histology of hepatopancreas (HPC) also shows Mp-fed groups has restores the normal structure. Conclusion: The study demonstrated that Mp enriched Ar in diet have a potentially role in ISt and an effective antioxidant by regulating infectious stress against vibriosis.
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Effect of Types Isolated Lactic Acid Bacteria on Hematocrit and Differential Leukocytes Fingerling Common Carp (Cyprinus carpio L.) Infected with Aeromonas hydrophila bacteria

72%
Shabirah A., Rosidah R., Mulyani Y., Lili W.
World News of Natural Sciences
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2019
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vol. 24
22-35
EN
This study aims to see the condition of hematocrit levels and differential leukosit after immersion in LAB isolated and after in vivo, using Aeromonas hydrophila bacterium. LAB isolated were obtained from the organs of the common carp intestine. The study used a Completely Randomized Design (RAL) consisting of four treatments and three replications. The treatment used was immersion of test fish for 24 hours in several isolates of LAB with a density of 108, namely A: without LAB isolate (control), B: isolate CcB7, C: isolate CcB8, D: isolate CcB15. Immersion was done three times, with a frequency of seven days. Parameters observed included hematocrit levels, leukocyte differentiation and survival rate. The results showed that immersion in LAB isolate CcB15 will enhance the differential leukocytes and hematocrit levels that serve as indicators of carp body resistance, which is shown by high levels of white and red blood cells.
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