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Adhesive and hydrophobic properties of the selected LAB isolated from gastrointestinal tract of farming animals

100%
Grajek K., Sip A., Foksowicz-Flaczyk J., Dobrowolska A., Wita A.
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2016
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vol. 63
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issue 2
311-314
EN
The aim of this study was to determine the degree of adhesion and hydrophobicity of some strains of lactic acid bacteria (LAB) with proven antagonistic properties against pathogenic bacteria. Studies were performed using-LAB strains isolated from the gastrointestinal tract of calves and piglets. These strains exhibited an antibacterial activity against pathogenic strains of Clostridium perfringens and Escherichia coli. Cell adhesiveness was examined in relation to the porcine and bovine mucin. Our analyses had shown that the tested microorganisms demonstrated a degree of adhesion in the range of 32.00-40.00% for strains isolated from calves, and 34.00-40.00% for strains isolated from pigs. The hydrophobicity of tested bacteria was in the range of 31.00%-44.00% for strains isolated from pigs, and 26.00%-42.00% for strains obtained from calves. The best mucin adhesion ability was found for bacterial strains belonging to the Enterococcus genus isolated from calves. Taking into account porcine isolates, the best adhesion was observed for the Leuconostoc bacterial strains. Among tested strains, the highest hydrophobicity, measured in relation to hexadecane, was recorded for the bacterial strains belonging to the Leuconostoc sp. isolated from piglets and a of Lactobacillus sp. bacterial strain isolated from calves.
2
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Evaluation of hydrophobicity and quantitative analysis of biofilm formation by Alicyclobacillus sp.

88%
Tyfa A., Kunicka-Styczyńska A., Zabielska J.
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2015
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vol. 62
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issue 4
785-790
EN
Alicyclobacillus sp. are acidothermophilic bacteria frequently contaminating fruit based products (juices and juice concentrates). These sporulating bacteria are able to survive at elevated temperatures and highly acidic environments which causes difficulties in their removal from industrial environments. Although numerous literature data examine Alicyclobacillus sp. presence in fruit based products and methods of their elimination, there is still a limited knowledge on ability of these bacteria to adhere to abiotic surfaces. Therefore, the objective of this study was to determine Alicyclobacillus sp. cells' hydrophobicity and capability of biofilm formation on a glass surface. The degree of cells hydrophobicity, according to Microbial Adhesion to Hydrocarbon (MATH) and Salt Aggregation Test (SAT), was investigated for eleven environmental isolates from natural Polish habitats, identified as Alicyclobacillus sp., and a Alicyclobacillus acidoterrestris DSM 3922 reference strain. The dynamics of biofilm formation within 3-day incubation on a glass surface was evaluated and quantified by a plate count method both, for cultures with and without agitation. All of the bacterial strains tested expressed ability to colonize a glass surface and four environmental isolates were classified as fast-adherent strains. The mature biofilm structures were predominantly formed after 48 hours of incubation. Dynamic culturing conditions were observed to accelerate the biofilm formation. The majority of strains expressed a moderate hydrophobicity level both, in SAT (41.7%) and MATH-PBS (75.0%), as well as MATH-PUM (91.7%) tests. However, no correlation between hydrophobicity and cell adherence to a glass slide surface was observed.
3
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Effect of surfactants on the flotation performance of low-rank coal by particle sliding process measurements

75%
Wang S., Tao X.
Gospodarka Surowcami Mineralnymi
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2018
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vol. 34
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issue 1
4
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Droplet impact in icing conditions – experimental study for WE 540

71%
Lizer T., Remer M., Sobieraj G., Psarski M., Pawlak D., Celichowski G., Lizer T., Remer M., Sobieraj G., Psarski M., Pawlak D., Celichowski G.
Archive of Mechanical Engineering
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2017
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vol. 64
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issue 2
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