The work describes purification and biochemical characterization of two inducible antimicrobial peptides from the hemolymph of Galleria mellonella. The peptides were isolated by a sequence of reversed-phase chromatography steps from the hemolymph of larvae immunized with viable bacteria. The first peptide is a member of the cecropin family while the second one is rich in proline residues and has a unique sequence.
The educational publication contains microphotographs and description of histological preparations of the structure of marbled crayfish. The above materials may be used both for carrying out laboratory work on disciplines "Histology", "Cytology", "Cell Biology", "Special Practice", and for self-study of relevant educational topics. Designed for specialists in the field of hydrobiology and histology, students and graduate students of institutions of higher education who studying in the field of "091 Biology", "207 Water bioresources and aquaculture" and "162 Biotechnology and bioengineering". The publication contains the results of studies conducted by President’s of Ukraine grant for competitive projects Ф75/142 «The reproductive potential of invasive species of Dnieper region reservoirs and their impact on bioproductivity formation» (№ 0118U006319) of the State Fund for Fundamental Research.
Main thiols and disulfides were determined in the hemolymph of the Jamaican field cricket Gryllus assimilis at various developmental stages. On the basis of these data, redox potentials of the glutathione, cysteine and homocysteine redox systems were calculated. The concentrations of all thiols studied decreased during development (at a stage of 6 molts) with respect to young crickets, and increased again in adult insects. Redox potentials of the glutathione and cysteine systems increased from values of -131.0±5.6 mV and -86.9±17.1 mV, respectively in young crickets to -58.0±3.6 mV and -36.1±4.2 mV, respectively, at the stage of 6 molts and decreased to values of -110.4±24.8 mV and -66.3±12.2 mV, respectively, in adult insects. Redox potentials of the glutathione and cysteine systems in the hemolymph of young and adult insects were similar to those reported for human plasma.
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