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ENCAPSULATION OF CENTAURIUM ERYTHRAEA RAFN – AN EFFICIENT METHOD FOR REGENERATION OF TRANSGENIC PLANTS

100%
Piątczak E., Wysokińska H.
Acta Biologica Cracoviensia s. Botanica
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2013
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vol. 55
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issue 2
2
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Antioxidant Profiles and Selected Parameters of Primary Metabolism in Physalis Ixocarpa Hairy Roots transformed by two Agrobacterium Rhizogenes Strains

86%
Bergier K., Kuźniak E., Wielanek M., Królicka A., Skłodowska M.
Acta Biologica Cracoviensia s. Botanica
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2012
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vol. 54
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issue 1
3
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Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.

86%
Długosz M., Wiktorowska E., Wiśniewska A., Pączkowski C.
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2013
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vol. 60
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issue 3
467-473
EN
In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode Induced Kinase) gene. The efficiency of induction of hairy roots reached 33.8% for cotyledons and 66.6% for hypocotyls together for both transformation experiments. Finally, eight control and nine modified lines were established as a long-term culture. The hairy root cultures showed the ability to synthesize oleanolic acid mainly (97%) as glycosides; control lines contained it at the average 8.42 mg · g-1 dry weight in tissue and 0.23 mg · dm-3 in medium; modified lines: 4.59 mg · g-1 for the tissue, and 0.48 mg · dm-3 for the medium. Additionally lines showed high positive correlation between dry/fresh weight and oleanolic acid concentration in tissue. Using the Killiani mixture in acidic hydrolysis of oleanolic acid glycosides released free aglycones that were partially acetylated in such conditions.
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