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1

Surgeons' and Hematologists' Decisions on Splenectomy in the Treatment of Congenital Spherocytosis

100%

Dadan J., Róg M., Wojskowicz P., Iwacewicz P., Safiejko K., Jaromin J., Róg-Makal M., Puchalski Z.

Polish Journal of Surgery

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2008

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vol. 80

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issue 3

146-152

EN

The aim of the study was to present and discuss the results of surgical treatment of congenital spherocytois in our department.Material and methods. Between 1994 and 2001 in the Department of General and Endocrinological Surgery at the Medical University in Białystok, 14 surgical excisions of enlarged spleens were performed because of congenital spherocytosis. The patients included five men and nine women aged between 17 and 61.Results. Diagnosis; non-surgical treatment and principles governing the decicion to use surgical intervention were evaluated by the Department of Hematology. Histopathological examinations for all cases confirmed the diagnosis of congenital spherocytosis.Conclusions. The undertaking of carefully considered procedures by the surgeons and hematologists in our departments resulted in propitious results in all the cases presented.

2

A New Method for Assessing Haemolysis in a Rotary Blood Pump Using Large Eddy Simulations (LES)

100%

Szwast M., Moskal A., Piątkiewicz W.

Chemical and Process Engineering

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2017

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vol. 38

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issue 2

3

Interaction of unmodified and partially silylated nanosilica with red blood cells

88%

Gun’ko V., Galagan N., Grytsenko I., Zarko V., Oranska O., Osaulenko V., Bogatyrev V., Turov V.

Open Chemistry

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2007

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vol. 5

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issue 4

951-969

EN

Interaction of red blood cells (RBCs) with unmodified and partially (50%) silylated fumed silica A-300 (nanosilica)was studied by microscopic, XRD and thermally stimulated depolarisation current (TSDC) methods. Nanosilica at a low concentration C A-300C A-300 = 1 wt% all RBCs transform into shadow corpuscles because of 100% haemolysis. Partial (one-half) hydrophobization of nanosilica leads to reduction of the haemolytic effect in comparison with unmodified silica at the same concentrations. A certain portion of the TSDC spectra of the buffered suspensions with RBC/A-300 is independent of the amounts of silica. However, significant portions of the low-and high-temperature TSDC bands have a lower intensity at C A-300 = 1 wt% than that for RBCs alone or RBC/A-300 at C A-300 = 0.01 wt.% because of structural changes in RBCs. Results of microscopic and XRD investigations and calculations using the TSDC-and NMR-cryoporometry suggest that the intracellular structures in RBCs (both organic and aqueous components) depend on nanosilica concentration in the suspension. [...]

4

Effect of carboxymethylated pyridoindoles on free radical-induced haemolysis of rat erythrocytes in vitro

75%

Juskova M., Snirc V., Krizanova L., Stefek M.

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2010

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vol. 57

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issue 2

153-156

EN

Recently novel carboxymethylated pyridoindoles, analogues of the efficient chain-breaking antioxidant stobadine, have been designed, synthesised and characterised as bifunctional compounds with joint antioxidant/aldose reductase inhibitory activities with the potential of preventing diabetic complications. The critical property for the efficacy of the novel aldose reductase inhibitors in vivo is their ability to penetrate into target tissues. In this study, the issue was addressed by measuring the antioxidant activity of compounds 1 [(2-benzyl-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole-8-yl)-acetic acid] and 2 [(±)-2-benzyl-(4a,9b)-cis-1,2,3,4,4a,9b-hexahydro-1H-pyrido[4,3-b] indole-8-yl acetic acid] in the cellular system of intact erythrocytes exposed to peroxyl radicals generated by thermal degradation of the azoinitiator 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH) in vitro. Isolated washed rat erythrocytes were incubated in the presence of the azoinitiator AAPH and the compounds tested for increasing periods of time up to 4 h at 37 °C. The degree of haemolysis was determined by absorbance of the haemoglobin released. The onset of AAPH-induced haemolysis was found to be shifted from the starting zero point by the time interval assigned as a lag period. In the presence of the compounds studied the lag period was prolonged significantly. The free radical-initiated haemolysis was retarded by the compounds studied with decreasing efficiency: stobadine > compound 1 ~ Trolox > compound 2. The results have demonstrated an antioxidant activity of the novel carboxymethylated pyridoindoles developed as potential agents for multitarget pharmacology of diabetic complications.

5

Gemini (dimeric) surfactant perturbation of the human erythrocyte.

63%

Dubnièková M., Bobrowska-Hägerstrand M., Söderström T., Igliè A., Hägerstrand H.

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2000

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vol. 47

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issue 3

651-660

EN

We studied the ability of di-cationic gemini surfactantsdi (amphiphiles), i.e. 1,4-butanediammonium-N,N-dialkyl-N,N,N',N'-tetramethyl bromides (Di-Cm-di-QAS (s = 4), where m = 8,11,13,16 and s = the number of alkyl groups in the spacer) to induce shape alteration, vesiculation, haemolysis and phosphatidylserine exposure in human erythrocytes, and to protect erythrocytes against hypotonic haemolysis. At high sublytic concentrations the Di-Cm-di-QAS (s = 4) amphiphiles rapidly induced echinocytic (spiculated) shapes and a release of exovesicles, mainly in the form of tubes, from the cell surface. Following 60 min incubation erythrocytes were sphero-echinocytic and a few cells with invaginations/endovesicles were observed. No phosphatidylserine exposure was detected. The haemolytic potency increased with an increase of the alkyl chain length. At sublytic concentrations the Di-Cm-di-QAS (s = 4) amphiphiles protected erythrocytes against hypotonic haemolysis. It is suggested that the Di-Cm-di-QAS (s = 4) amphiphiles perturb the membrane in a similar way as single-chain cationic amphiphiles, but that they do not easily translocate to the inner membrane leaflet.

Refine search results

Surgeons' and Hematologists' Decisions on Splenectomy in the Treatment of Congenital Spherocytosis

A New Method for Assessing Haemolysis in a Rotary Blood Pump Using Large Eddy Simulations (LES)

Interaction of unmodified and partially silylated nanosilica with red blood cells

Effect of carboxymethylated pyridoindoles on free radical-induced haemolysis of rat erythrocytes in vitro

Gemini (dimeric) surfactant perturbation of the human erythrocyte.