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Ganoderma lucidum extract stimulates glucose uptake in L6 rat skeletal muscle cells

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Jung K. H., Ha E., Kim M.-J., Uhm Y. K., Kim H. K., Hong S.-J., Chung J.-H., Yim S.-V.

The effect of Ganoderma lucidum extract on glucose uptake was studied in L6 rat skeletal muscle cells. G. lucidum extract increased glucose uptake about 2-fold compared to control. The extract stimulated the activity of phosphatidylinositol (PI) 3-kinase which is a major regulatory molecule in the glucose uptake pathway. About 7-fold increased activity of a PI 3-kinase was observed after treatment with G. lucidum extract, whereas PI 3-kinase inhibitor, LY294002, blocked the G. lucidum extract-stimulated PI 3-kinase activity in L6 skeletal muscle cells. Protein kinase B, a downstream mediator of PI 3-kinase, was also activated by G. lucidum extract. We then assessed the activity of AMP-activated protein kinase (AMPK), another regulatory molecule in the glucose uptake pathway. G. lucidum extract increased the phosphorylation level of both AMPK α1 and α2. Activity of p38 MAPK, a downstream mediator of AMPK, was also increased by G. lucidum extract. Taken together, these results suggest that G. lucidum extract may stimulate glucose uptake, through both PI 3-kinase and AMPK in L6 skeletal muscle cells thereby contributing to glucose homeostasis.

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1 Acta Biochimica Polonica

1 2006

1 Chung J.-H.

1 Ha E.

1 Hong S.-J.

1 Jung K. H.

1 Kim H. K.

1 Kim M.-J.

1 Uhm Y. K.

1 Yim S.-V.

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Ganoderma lucidum extract stimulates glucose uptake in L6 rat skeletal muscle cells