We developed a fluorescence confocal microscope equipped with a hemispherical solid immersion lens (SIL) and apply it to study the optical properties of light-harvesting complexes. We demonstrate that the collection efficiency of the SIL-equipped microscope is significantly improved, as is the spatial resolution, which reaches 600 nm. This experimental setup is suitable for detailed studies of physical phenomena in hybrid nanostructures. In particular, we compare the results of fluorescence intensity measurements for a light-harvesting peridinin-chlorophyll-protein (PCP) complex with and without the SIL.
Since its first demonstration of functional imaging in small animals about a decade ago, photoacoustic tomography (PAT) has quickly become one of the fastest growing biomedical imaging modalities. Combining optical excitation with acoustic detection, PAT can provide detailed images of tissues deep in the body. While PAT technology continues to improve significantly, substantial efforts have also been made to develop multimodal PAT systems. These systems not only provide complementary information for more comprehensive characterization of tissue, they also generate data that can be used to further improve PAT reconstruction. This review will present current progress in multimodal PAT imaging, focusing on the technical aspects of integration and its applications in biomedicine.
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