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EN
The diluted ethanol solutions and fermentation broth (Saccharomyces cerevisiae) were separated by membrane distillation (MD). Hydrophobic macroporous (pore size 0.2 μm) capillary polypropylene membranes, Accurel PP V8/2 HF and Accurel PP S6/2, were used for these studies. The MD process can be successfully applied to remove the volatile components from the fermentation broth. Besides ethanol, propionic and acetic acids were moved from the broth to the distillate. Therefore, the course of the fermentation carried out in a membrane distillation bioreactor considerably accelerate its rate and increase the efficiency by a selective removal of fermentation products. It was found that the broth subjected to the separation did not affect the hydrophobic properties of the polypropylene membrane assembled in the MD modules.
EN
Sweet sorghum juice and traditional ethanol substrate i.e. sugarcane molasses were used for ethanol production in this work. At the end of the fermentation process, the sweet sorghum juice yielded more ethanol with higher ethanol concentration compared to sugarcane molasses in all experiments. The sweet sorghum juice had higher cell viability at high ethanol concentrations and minimum sugar concentration at the end of the fermentation process. The ethanol concentration and yield were 8.9% w/v and 0.45 g/g for sweet sorghum in 80 h and 6.5% w/v and 0.37 g/g for sugarcane molasses in 60 h, respectively. The findings on the physical properties of sweet sorghum juice revealed that it has better physical properties compared to sugarcane molasses, resulting to enhanced performance of sweet sorghum juice for ethanol production
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Microbial amylases: A review

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EN
Microbial amylases are enzymes produced by microorganisms to hydrolyze starch. There are three types of microbial amylases: alpha-amylase, beta-amylase and glucoamylase. Each of these amylases has a unique way of acting on starch to yield simple glucose monomers. Microorganisms, plants, and animals are sources of amylases, but much attention is given to microorganisms since the amylases produced by them have greater thermal stability and give rise to different sugar profiles, thus meeting industrial demands. Two major groups of microorganisms play pivotal role in amylase production: bacteria and fungi. Starch is the substrate used in amylase production. Between the two fermentation processes used in amylase production (i.e. submerged fermentation and solid state fermentation), the latter is more advantageous as it saves cost, generates little effluent, and has high volumetric productivity. Microbial amylases are greatly applied in pharmaceutical, food, chemical, paper and distilling industries.
EN
Substitution of fossil fuels with alternative energy carriers has become necessary due to climate change and fossil fuel shortages. Fermentation as a way of producing biohydrogen, an attractive and environmentally friendly future energy carrier, has captured received increasing attention in recent years because of its high H2 production rate and a variety of readily available waste substrates used in the process. This paper discusses the state-of-the-art of fermentative biohydrogen production, factors affecting this process, as well as various bioreactor configurations and performance parameters, including H2 yield and H2 production rate.
EN
1,3-propanediol is a promising monomer with many applications and can be produced by bioconversion of renewable resources. The separation of this product from fermentation broth is a difficult task. In this work, the application of cation exchange resin for the separation of 1,3-propanediol from model aqueous solution was examined. The best effect of separation of 1,3-propanediol from glycerol using sorption method was obtained for H+ resin form, although the observed partition coefficient of 1,3-propanediol was low. On the basis of the results of the sorption of 1,3-propanediol, the ionic forms of the resin were selected and used in the next experiments (H+, Ca2+, Ag+, Na+, Pb2+, Zn2+). The best results in ion exchange chromatography were obtained for cation exchange resin in H+ and Ca2+ form. The use of smaller particle size of resin and a longer length of the column allows to obtain better separation of mixtures.
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vol. 44
89-110
EN
The use of acids in the hydrolysis of starch for industrial and domestic purpose have become relatively unstable, expensive, difficult to control and even harmful to users over the years. This study aimed at isolating and characterizing alpha -amylase producing yeasts and investigate the effects of different cultural and physico-chemical parameters on the production of alpha- amylase. A total of forty- three (43) yeast strains were isolated from different fermented foods such as ogi, wara, palm wine and kunu. The isolates (OG9 and WR4) had the highest activity and were identified as: Candida tropicalis strain IFM 63517 and Candida tropicalis strain CMC 1836 respectively. Fermentation parameters such as incubation period, pH, temperature, effect of surfactants, carbon and nitrogen source were optimized using submerged fermentation. The optimum pH, temperature and incubation period for enzyme production were: 5.0, 30 °C and 48 hours respectively. Of the carbon sources, soluble starch at 1% concentration was observed to be the best carbon source for enzyme production using the two yeasts. Yeast extract at 1% concentration was ideal nitrogen source for the yeasts. Surfactants Tween-80 was most effective for enhancement of alpha amylase production. Cacl2 supported the highest alpha amylase activity for both strains. This study revealed that favourable fermentation conditions and the selection of suitable growth parameters played key roles in the production of alpha amylase by Candida tropicalis. Soluble starch is a good substrate for alpha amylase production. Alpha- amylase producing yeast strains with increasing enzyme activities at high temperature are promising candidates for industrial application.
EN
The ethanol production industry is a fast growing branch of the economy in many countries, and there is a rich tradition of spirit beverage production of many unique drinks such as Polish vodka and Starka or Irish and Scotch whisk(e)y, all of which have unique organoleptic features. This variety is possible thanks to different raw materials used for production such as rye, barley or corn and potatoes, as well as technological solutions developed over the generations of manufacturing. Rye deserves a closer look due to its low growth requirements and many different uses as well as its long tradition of cultivation, especially in Poland. On the other hand, manufacturers are currently interested in using new, original raw materials for the production of so-called craft alcohols. Buckwheat is an example of a raw material that can be successfully used in the production of original spirits.
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EN
Basing on long-standing cyclic measurements of sludge-originated biogas composition and considering statistical analysis of their results, a regression model describing time variation of biogas desulfurization using bog iron ore has been developed. The model was verified by theoretical calculations and results from laboratory examinations of the sorbent. It was also used to estimate the depletion time and sorption capacity of the bed and to determine the demand index for bog ore.
PL
Na bazie wieloletnich (2003-2009), cyklicznych pomiarów składu biogazu powstającego z osadów ściekowych, na podstawie analizy statystycznej ich wyników opracowano model regresyjny opisujący czasowy charakter zmienności skuteczności odsiarczania biogazu za pomocą rudy darniowej. Model został zweryfikowany z wykorzystaniem obliczeń teoretycznych i wyników badań laboratoryjnych sorbentu. Za jego pomocą oszacowano czas wyczerpania i pojemność sorpcyjną złoża oraz wyznaczono wskaźnik zapotrzebowania na rudę darniową.
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