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Lipopolysaccharide (LPS, endotoxin) of Proteus bacteria-chemical structure, serological specificity and the role in pathogenicity

100%

Różalski A., University of Łódź I. o. M. a. I.

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

2008

vol. 4

Lipopolysaccharides (LPS) of Gram-negative bacteria are composed of three regions: О-specific chain (OPS), the core oligosaccharide and lipid A. All three regions of Proteus LPS were studied. The differences in the structure of OPS serve as the basis for the serological classification of Proteus strains. The serological classification scheme of these bacteria currently consists of 76 serogroups. The structural diversity of the core region is characteristic for Proteus sp. and distinguishes this genus from other bacteria. In this paper the results of structural, immunochemical and serological studies of all three regions o f Proteus LPS, as well as a function of LPS as endotoxin and its role in the formation of urinary stones, swarming phenomenon and bacterial growth in biofilm are reported.

Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization.

100%

Budryk M., Cichoń T., Szala S.

The purpose of this study was to investigate the effect of endotoxin presence in plasmid DNA preparations on the efficiency of transfection achieved in vivo with B16(F10) and Renca tumors and to determine transgene localization. Our data show that endotoxin markedly decreases the efficiency of transfection. Furthermore, the transgene transferred in vivo can be found in both neoplastic and normal (most likely myofibroblast) cells lying in proximity of the administration site.

Comparison of serological specificity of anti-endotoxin sera directed against whole bacterial cells and core oligosaccharide of Escherichia coli J5-tetanus toxoid conjugate*.

88%

Lukasiewicz J., Jachymek W., Niedziela T., Malik-Gebicka M., Dzieciatkowska M., Lugowski C.

2002

vol. 49

issue 3

721-734

The rough mutants of Gram-negative bacteria are widely used to induce protective antisera but the nature of the target epitope for such antibodies is not precisely defined. Endotoxin is one of several antigens present on the surface of bacterial cells, which are able to elicit specific antibodies. We studied the specificity of antibodies produced against a conjugate of E. coli J5 endotoxin core oligosaccharide with tetanus toxoid. The use of chemically defined antigen for immunisation excludes the possibility of production of antibodies against other cell surface antigens. A comparison of this monospecific anti-endotoxin serum with antiserum against E. coli J5 whole cells was performed in order to distinguish the role that endotoxin core oligosaccharide plays in the interaction with humoral host defences from that of other potentially important Gram-negative bacterial surface antigens. The reactivity of both sera with smooth and rough lipopolysaccharides was determined in ELISA, immunoblotting and by flow cytometry. Both antisera reacted with similar specificity with most lipopolysaccharides of identical or related core type. Less distinct reactions with endotoxins of the antibacterial serum in comparison with the anti-conjugate serum were found in all serological tests. LPS of E. coli O100 that showed the strongest reactions with both sera was used to stimulate IL-6, TNFα and nitric oxide production by the J-774A.1 cell line. Both sera were used to inhibit that stimulation and no inhibitory effects of the examined sera in comparison with non-immune serum were observed.

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2 Acta Biochimica Polonica

1 Acta Universitatis Lodziensis. Folia Biologica et Oecologica

1 2008

1 2002

1 2001

1 Budryk M.

1 Cichoń T.

1 Dzieciatkowska M.

1 Jachymek W.

1 Lugowski C.

1 Lukasiewicz J.

1 Malik-Gebicka M.

1 Niedziela T.

1 Różalski A.

1 Szala S.

1 University of Łódź I. o. M. a. I.

Search results

Lipopolysaccharide (LPS, endotoxin) of Proteus bacteria-chemical structure, serological specificity and the role in pathogenicity

Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization.

Comparison of serological specificity of anti-endotoxin sera directed against whole bacterial cells and core oligosaccharide of Escherichia coli J5-tetanus toxoid conjugate*.