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  1. 1 Acta Biochimica Polonica

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  1. 1 2003

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  1. 1 Bardowski J.

  2. 1 Kowalczyk M.

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Overproduction and purification of the CcpA protein from Lactococcus lactis.

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Kowalczyk M., Bardowski J.
|
2003
|
vol. 50
|
issue 2
455-459
EN
In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6×His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 Μg/ml ampicillin at 37°C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.
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