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Desorption/ionization on silicon for small molecules: a promising alternative to MALDI TOF.

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Kraj A., Dylag T., Gorecka-Drzazga A., Bargiel S., Dziuban J., Silberring J.

A method has been developed for laser desorption/ionization of catecholamines from porous silicon. This methodology is particularly attractive for analysis of small molecules. MALDI TOF mass spectrometry, although a very sensitive technique, utilizes matrices that need to be mixed with the sample prior to their analysis. Each matrix produces its own background, particularly in the low-molecular mass region. Therefore, detection and identification of molecules below 400 Da can be difficult. Desorption/ionization of samples deposited on porous silicon does not require addition of a matrix, thus, spectra in the low-molecular mass region can be clearly readable. Here, we describe a method for the analysis of catecholamines. While MALDI TOF is superior for proteomics/peptidomics, desorption/ionization from porous silicon can extend the operating range of a mass spectrometer for studies on metabolomics (small organic molecules and their metabolites, such as chemical neurotransmitters, prostaglandins, steroids, etc.).

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1 Acta Biochimica Polonica

1 2003

1 Bargiel S.

1 Dylag T.

1 Dziuban J.

1 Gorecka-Drzazga A.

1 Kraj A.

1 Silberring J.

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Desorption/ionization on silicon for small molecules: a promising alternative to MALDI TOF.