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In vitro effect of pentoxifylline and lisofylline on deformability and aggregation of red blood cells from healthy subjects and patients with chronic venous disease

100%
Słoczyńska K., Kózka M., Pękala E., Marchewka A., Marona H.
|
2013
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vol. 60
|
issue 1
129-135
EN
Purpose. The aim of the study was to assess the in vitro potency of pentoxifylline (PTX) and one of its most active metabolites lisofylline (LSF) to improve rheological properties of red blood cells (RBC) from healthy individuals and patients with chronic venous disease (CVD). Additionally, the study aimed to compare the effects of PTX and LSF on RBC deformability and aggregation. Methods. Blood samples were collected from healthy volunteers (antecubital vein) and from CVD patients (varicose and antecubital vein). Deformability and aggregation of RBC were assessed using Laser-assisted Optical Rotational Cell Analyser (LORCA). Results. PTX and LSF increased RBC elongation significantly. Additionally, RBC incubation with PTX resulted in a marked decrease in RBC aggregation. PTX reduced the tendency towards the formation of RBC aggregates and of their stability. The beneficial effect of PTX on RBC aggregation was most apparent for those cells whose aggregation tendency and aggregate stability was the greatest. Conclusions. In vitro addition of PTX or LSF effectively increased deformability of RBC from healthy donors and patients with CVD. Thus, LSF may contribute to the in vivo hemorheological effects of pentoxifylline. On the other hand, there was no significant effect of LSF on aggregation of RBC in vitro. Hence, LSF has no contribution to this particular effect of PTX. Additionally, the present study demonstrated the use of RBC with impaired deformability and aggregation for the evaluation of in vitro rheological activity of xenobiotics.
2
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A Pen206 Borehole Jack Suitability Assessment for Rock Mass Deformability Determination

86%
Pierszalik R., Rajwa S., Walentek A., Bier K., Pierszalik R., Rajwa S., Walentek A., Bier K.
Archives of Mining Sciences
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2020
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vol. 65
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issue 3
3
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Influence of addition of shredded rubber waste on deformability of binder-bound anthropogenic material mixtures

86%
Walotek K., Bzówka J., Ciołczyk A., Walotek K., Bzówka J., Ciołczyk A.
Archives of Civil Engineering
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2021
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vol. 67
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issue 4
4
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Mathematical Simulation of Reinforced Concrete Beams with the External Reinforcement in the Form of Steel Cut and Stretchy Sheet

72%
Burchenya S., Roman S.
Teka Commission of Motorization and Power Industry in Agriculture
|
2016
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vol. 16
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issue 3
5
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Kliniczne zastosowania laserowo-optycznego rotacyjnego analizatora krwinek czerwonych LORCA

72%
Słowińska L., Monkos K.
Annales Academiae Medicae Silesiensis
|
2010
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vol. 64
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issue 3-4
42-47
EN
Laser-assisted Optical Rotational Cell Analyser LORCA is a precise instrument capable to investigation of aggregations and deformability of erythrocytes. In the paper the action principles of the instruments is presented and the measured quantities such as the aggregation amplitude, aggregation half time, aggregation index and elongation index are defi ned. A comparison of mean values of those quantities for the control group and groups of patients with obesity (body mass index – BMI > 35), pancreas tumor, myocardial infarction, obstructive sleep apnea, age-related macular degeneration and diabetes mellitus of the type 2 is made.
PL
Laserowo-optyczny rotacyjny analizator krwinek czerwonych LORCA jest precyzyjnym przyrządem służącym do badania agregacji i odkształcalności erytrocytów. W pracy przedstawiono zasadę działania tego przyrządu oraz zdefiniowano wielkości przez niego mierzone, takie jak amplituda agregacji, czas połowicznego odtwarzania agregacji, indeks agregacji oraz indeks elongacji. Porównano średnie wartości tych wielkości dla grupy kontrolnej osób zdrowych oraz grup chorych na otyłość (body mass index – BMI > 35), raka trzustki, zawał serca, obturacyjny bezdech we śnie, zwyrodnienie plamki żółtej związane z wiekiem oraz cukrzycę typu 2.
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