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Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

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Pupek M., Lemańska-Perek A., Jasonek J., Kątnik-Prastowska I.

2010

vol. 57

issue 3

333-337

Three monoclonal antibodies specific to the central cell-binding and the C- and N-terminal domains of fibronectin (FN) were used to test antigenic epitope accessibility on human plasma and cerebrospinal fibronectins. In the plasma group, the mean N-terminal FN domain immunoreactivity was about one fourth that of the cell-binding and C-terminal domains, whereas in cerebrospinal fluid they were nearly equal. In the presence of 0.5-6 M urea N-terminal domain immunoreactivity in the plasma increased 3-6-fold, but it decreased 0.7-3-fold in the cerebrospinal fluid. Analysis of fibronectin domain immunoreactivities of the cell-binding and N-terminal domains by a panel of specific monoclonal antibodies may reveal N-terminal fibronectin domain accessibility for reaction with biological partner ligand(s) and/or processes in which FN could be implicated. Such determinations may have important clinical implications.

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1 Acta Biochimica Polonica

1 2010

1 Jasonek J.

1 Kątnik-Prastowska I.

1 Lemańska-Perek A.

1 Pupek M.

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Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains