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1
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Molecular identification of Babesia spp isolated from Polish cattle with asymptomatic protozoa infections

100%
Staniec M., Adaszek Ł., Buczek K., Winiarczyk S.
Polish Journal of Veterinary Sciences
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2018
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vol. 21
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issue 2
2
Content available remote

Genetic diversity in populations of Slovak Spotted cattle based on single nucleotide polymorphisms analyses

88%
Moravčíková N., Trakovická A., Navrátilová A.
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2013
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vol. 60
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issue 4
807-810
EN
The aim of this study was to identify SNPs in leptin (LEP), leptin receptor (LEPR) and growth hormone (GH) genes in order to analyze genetic diversity of Slovak Spotted cattle. The total numbers of blood samples were taken from 353 Slovak Spotted cows originating from four farms. Genomic DNA was isolated by phenol-chloroform extraction method and analyzed by PCR-RFLP method. After digestion with restriction, enzymes were detected in whole population of cow's alleles with frequency: LEP/Sau3AI A 0.84 and B 0.16 (±0.0152); LEPR/BseGI C 0.95 and T 0.05 (±0.0089) and GH/AluI L 0.70 and V 0.30 (±0.0188). Based on the observed vs. expected genotypes frequencies populations across loci were in Hardy-Weinberg equilibrium (P\>0.05). Predominant for SNP LEP/Sau3AI was AA genotype (0.70), for SNP LEPR/T945M CC genotype (0.91), and LL genotype (0.48) was most frequent for SNP GH/AluI. The observed heterozygosity of SNPs across populations was also transferred to the low or median polymorphic information content 0.24 (He 0.28), 0.08 (He 0.09) and 0.33 (He 0.47) for LEP, LEPR and GH genes, respectively. Within genetic variability estimating negative values of fixation indexes FIS (-0.09-0.05) and FIT (-0.07-0.03) indicating heterozygote excess were observed. The value of FST indexes (0.018-0.023) shows very low levels of genetic differentiation in allele frequencies of loci among evaluated subpopulations. The low values of genetic distances (0.0018-0.0159) indicated high genetic relatedness among animals in subpopulations caused probably by common ancestry used in breeding program at farms.
3
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CD4- and CD8-expressing cells found in the bovine and porcine anterior chamber of the eye

75%
Ziółkowski H., Maślanka T., Socha P., Ziółkowska N., Dąbrowski M., Małaczewska J.
Polish Journal of Veterinary Sciences
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2018
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vol. 21
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issue 2
4
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The influence of short-term selenitetriglycerides supplementation on blood selenium, and hepatic, renal, metabolic and hematological parameters in dairy cows

75%
Żarczyńska K., Sobiech P., Mee J., Illek J., Żarczyńska K., Sobiech P., Mee J., Illek J.
Polish Journal of Veterinary Sciences
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2020
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vol. 23
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issue 4
5
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The effect of DDE and dieldrin and their parental substances (DDT and aldrin) on PGE2 and PGF2α release from bovine endometrial explants collected during 120 to 180 days of the gestational period

75%
Mlynarczuk J., Wrobel M. H., Dobrzyn K., Mlynarczuk J., Wrobel M. H., Dobrzyn K.
Polish Journal of Veterinary Sciences
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2021
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vol. 24
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issue 1
6
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Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle

75%
Trakovická A., Moravčíková N., Kasarda R.
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2013
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vol. 60
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issue 4
783-787
EN
Leptin and leptin receptor genes are considered as production traits markers in dairy or beef cattle. The aim of this study was to verify the associations of polymorphisms in bovine LEP and LEPR genes with production and reproduction traits in Slovak Spotted and Pinzgau cows. Long-life production was evaluated: milk, protein, and fat yield and reproduction traits: age at first calving, calving interval, days open, and insemination interval. In total, 296 blood samples of Slovak Spotted and 85 hair roots samples of Pinzgau cows were analyzed. In order to detect LEP/Sau3AI (BTA 4, inron 2) and LEPR/T945M (BTA 3, exon 20) genotypes PCR-RFLP method was used. In Slovak Spotted and Pinzgau cows allele frequencies were 0.838/0.162 and 0.694/0.306 for A and B LEP variants, and 0.954/0.046 and 0.912/0.088 for C and T LEPR variants, respectively. For testing the associations between SNPs LEP/Sau3AI and LEPR/T945M and evaluated traits, the General Linear Model procedure in SAS Software was used. Statistical analysis showed that SNP LEP/Sau3AI significantly affected milk, protein and fat yield (P<0.05), and age at first calving (P<0.01) in analyzed population of cows. Statistically, SNP LEPR/T945M affected significantly calving interval (P<0.01) only. Results of our study suggest that especially leptin is a candidate gene, which influences mainly milk production traits and might be implemented in breeding strategies to improve the production performance of both analyzed cattle breeds.
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