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SYNTHESIS OF CHITOSAN AND CARBOXYMETHYL CHITOSAN HYDROGELS BY ELECTRON BEAM IRRADIATION

100%

Czechowska-Biskup R., Wach R., Stojek P., Kamińska M., Rosiak J. M., Ulański P.

Progress on Chemistry and Application of Chitin and its Derivatives

2016

vol. 21

27-45

Solutions of chitosan (CS) and carboxymethyl chitosan (CMCS) were subjected to irradiation by electron beam in presence of a crosslinking agent (poly(ethylene glycol) diacrylate - PEGDA) in order to produce carboxymethyl chitosan- and chitosan-based hydrogels. PEGDA macromonomer itself undergoes simultaneous polymerization and crosslinking either in neutral water or in acidic medium. Acidic solutions of chitosan of 0.5, 1 and 2% can be effectively crosslinked with PEGDA to form a gel. Although CMCS undergoes radiation-initiated crosslinking only at high concentration in water (over 10%), the presence of PEGDA in solution facilitated hydrogel formation even at lower concentration of CMCS. Formation of CS and CMCS hydrogels required irradiation doses lower than those needed for sterilization, i.e. 25 kGy, in some cases even as low as 200 Gy. Sol-gel analysis revealed relatively high gel fraction of obtained hydrogels, up to 80%, and good swelling ability. Both parameters can be easily controlled by composition of the initial solution and irradiation dose. Possible mechanisms of crosslinking reactions were proposed, involving addition of the polysaccharide macroradicals to a terminal double bond of PEGDA.

SYNTHESIS AND POTENTIAL CYTOTOXICITY EVALUATION OF CARBOXYMETHYL CHITOSAN HYDROGELS

86%

Kłosiński K. K., Girek M. K. G., Wach R. A., Czechowska-Biskup R., Arkuszewski P. T., Szymański P. B., Pasieka Z.

Progress on Chemistry and Application of Chitin and its Derivatives

2017

vol. 22

82-96

The aim of the research was to employ radiation to produce flexible carboxymethyl chitosan (CMCS) based hydrogels of uniform structure to characterise their swelling properties and cytocompatibility for potential applications as hydrogel wound dressings. CMCS in aqueous solution was irradiated with an electron beam in the presence of a poly(ethylene glycol) diacrylate (PEGDA) macromonomer as a crosslinker, at 12 different compositions, i.e. 3–20% CMCS, 3 and 5% PEGDA. The obtained hydrogels were subjected to sol–gel analysis. The amount of insoluble fraction (up to 100%) rose with an increase in the PEGDA/polysaccharide ratio. Moreover, the equilibrium degree of swelling, ca. 15 to 200 g of water per g of gel, which was higher for lower content of crosslinker, decreased with the delivered dose, which was associated with an increase in crosslinking density. The in vitro XTT cell viability assay (murine fibroblasts, L929 cell line) showed no significant cytotoxicity of CMCS gels.

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2 Progress on Chemistry and Application of Chitin and its Derivatives

1 2017

1 2016

2 Czechowska-Biskup R.

1 Arkuszewski P. T.

1 Girek M. K. G.

1 Kamińska M.

1 Kłosiński K. K.

1 Pasieka Z.

1 Rosiak J. M.

1 Stojek P.

1 Szymański P. B.

1 Ulański P.

1 Wach R.

1 Wach R. A.

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SYNTHESIS OF CHITOSAN AND CARBOXYMETHYL CHITOSAN HYDROGELS BY ELECTRON BEAM IRRADIATION

SYNTHESIS AND POTENTIAL CYTOTOXICITY EVALUATION OF CARBOXYMETHYL CHITOSAN HYDROGELS