Search results

1

Effect of cadmium on collagen content and solubility in rat bone.

100%

Galicka A., Brzóska M. M., Średzińska K., Gindzienski A.

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vol. 51

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issue 3

825-829

EN

The toxic action of cadmium in the bone tissue is known, but its mechanisms are still unexplained. We examined whether Cd influences collagen content and its solubility in the femoral bone of three-week-old female rats exposed to 5 or 50 mg Cd/l in drinking water. Non-cross linked collagen was extracted with 0.5 M acetic acid, and two acid-insoluble collagen fractions were extracted with pepsin and 4.0 M guanidine hydrochloride, respectively. SDS/PAGE showed the presence of two collagen types, I and V, in all three extracted fractions. Exposure of rats to Cd for 6 months increased the amount of acid-soluble collagens type I and V and decreased the level of acid-insoluble collagens. The amount of total collagen extracted from the bones of rats exposed to 50 mg Cd/l was reduced by about 14% as compared to control and those intoxicated with 5 mg Cd/l. The solubility of type I bone collagen (determined as the percentage of acetic-soluble fraction of total collagen) was increased 2.9- and 3.0-fold in rats intoxicated with 5 and 50 mg Cd/l, respectively. Similarly, the solubility of type V collagen was increased 2.3- and 2.7-fold, respectively. Our results indicate that Cd treatment affects bone collagen by decreasing its content and increasing its solubility.

2

Participation of migration paths of selected metals in bone intoxication of Upper Silesia inhabitants

88%

Brodziak-Dopierała B., Kwapuliński J., Wiechuła D., Sobczyk K.

Annales Academiae Medicae Silesiensis

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2017

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vol. 71

275-281

EN

INTRODUCTION: The aim of the paper was to analyze the content of cadmium, manganese, chromium, lead, zinc and copper in the bone tissue of people living in cities in the Upper Silesia Industrial District (Bytom, Katowice, Chorzów, Siemianowice Śląskie, Świętochłowice). It also specifies the relationship between the content of selected metals in the environment and in bone tissue. MATERIAL AND METHODS: The sample material was collected at the Municipal Hospital in Siemianowice Ślaskie. The study group amounted to 53 heads of femur, including 43 from women and 10 from men. The studied tissues were taken intraoperatively during hip replacement procedures (endoprosthetics). The content of cadmium, manganese, chromium, lead, zinc and copper was determined by atomic absorption spectrophotometry using a Pye Unicam SP-9 apparatus. RESULTS: The average content of elements in the femoral heads in an increasing series is as follows: Cd 0.16 < Ni 0.85 < Mn 0.87 < Cu 3.77 < Cr 5.37 < Zn 82.42 (µg/g). CONCLUSIONS: The metal intoxication of bones sourced in suspended particulates and dust emitted from soil into the air mainly concerned the residents of Katowice. Referring this information to the obtained results, the highest contents of cadmium, copper, and chromium in the bone tissue of the inhabitants of this city can be confirmed.

PL

WSTĘP: Celem pracy była analiza zawartości kadmu, manganu, chromu, ołowiu, cynku i miedzi w tkance kostnej osób zamieszkujących miasta na terenie Górnego Śląska (Bytom, Katowice, Chorzów, Siemianowice Śląskie, Świętochłowice). Określono również ponadto zależność między zawartością wybranych metali w środowisku i w tkance kostnej. MATERIAŁ I METODY: Materiał pobierano w Szpitalu Miejskim w Siemianowicach Śląskich. W grupie badanej poddano analizie 53 głowy kości udowej, w tym 43 od kobiet i 10 od mężczyzn. Próbki tkanki kostnej zostały pobrane w trakcie endoprotezoplastyki stawu biodrowego. Badane tkanki pozyskiwane były śródoperacyjnie w trakcie zabiegów endoprotezoplastyki stawu biodrowego. Zawartość kadmu, manganu, chromu, ołowiu, cynku i miedzi oznaczano za pomocą metody atomowej spektrofotometrii absorpcyjnej za pomocą aparatu Pye Unicam SP-9. WYNIKI: Średnia zawartość pierwiastków w tkankach głowy kości udowej przedstawiała się następująco: Cd 0,16 < Ni 0,85 < Mn 0,87 < Cu 3,77 < Cr 5,37 < Zn 82,42 (µg/g). WNIOSKI: Intoksykacja kości metalami z pyłu zawieszonego oraz pyłu wyemitowanego z gleby do powietrza dotyczy głównie mieszkańców Katowic. Porównując te informacje z uzyskanymi wynikami potwierdza się największą zawartość takich metali, jak kadm, miedź, chrom, w tkance kostnej mieszkańców tego miasta.

3

New generation of phosphate fertilizer from bones, produced by bacteria

88%

Wyciszkiewicz M., Saeid A., Górecki H., Chojnacka K.

Open Chemistry

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2015

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vol. 13

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issue 1

EN

In this article, the phytotoxicity of biofertilizer produced from bones and its utilitarian properties are presented. Biofertilizer was obtained from bones in a solubilization process of phosphates conducted by bacteria Bacillus megaterium. Two in vivo tests were used for examination of the utilitarian properties of the biofertilizer: a hydroponic and a germination test. The experiment was performed using three groups of plants and four replications: group 1 − not treated (control 1), group 2 − with a conventional fertilizer (control 2), and group 3 – with the biofertilizer (experimental group). In the hydroponic tests, the best growth parameters were found for the samples where the biofertilizer was applied. The greater dry mass of plants was observed for plants collected from this group compared to the control 1 group and the group where the commercial fertilizer were used. In the case of the plant length and the intensity of green color, statistically significant differences were found. The utilitarian properties of the biofertilizer, evaluated from a germination test, were similar to those of the classical fertilizer. Statistically significant differences were found between the mass and intensity of green color of the experimental group (with the biofertilizer) and the control 1 group.

4

Method of skeletal system modelling

71%

Olszewski H., Wojnicz W., Wittbrodt E., Olszewski H., Wojnicz W., Wittbrodt E.

Archive of Mechanical Engineering

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2013

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vol. 60

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issue 3

5

Остеостимулююча дія полівалентного орального гелю на стан кісткової тканини нижньої щелепи щурів з преднізолоновим пародонтитом

63%

Levitsky A. P., Borysenko A. V., Stolyar V. G.

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issue 6

21-30

EN

Osteostimulatory Effect of Polyvalent Oral Gel on the Bone Tissue of the Rats’ Lower Jaw with Prednisolon Periodontitis A. P. Levitsky, A. V. Borysenko, V. G. Stolyar Objective. To determine osteostimulating effect of polyvalent oral gel on the bone when modeling periodontal prednisolon periodontitis. Materials and Methods. We used polyvalent oral gel containing osteovit (calcium citrate, soy isoflavones, zinc sulfate and vitamin D3), propolis tincture, metrogil-dent, chlorhexidine and silica, which was applied to the oral mucosa in applications during two weeks to rats which recieved prednisolon-containing feed. Altogether, 32 rats. As a comparison drug we used mefenat. Results. The reduction of acid phosphatase activity and increase of the mineralizing index, increase and the ratio of calcium content calcium/protein in the bone tissue under the influence of a polyvalent gel was achieved. Conclusion. Proposed gel has osteostimulating effect.

PL

ОСТЕОСТИМУЛИРУЮЩЕЕ ДЕЙСТВИЕ ПОЛИВАЛЕНТНОГО ОРАЛЬНОГО ГЕЛЯ НА СОСТОЯНИЕ КОСТНОЙ ТКАНИ НИЖНЕЙ ЧЕЛЮСТИ КРЫС С ПРЕДНИЗОЛОНОВЫМ ПАРОДОНТИТОМ А. П. Левицкий, А. В. Борисенко, В. Г. Столяр Поливалентный оральный гель (ПОГ), состоящий из остеовита, настойки прополиса, метрогил-дента, хлоргексидина и диоксида кремния, оказывает остеостимулирующее действие на костную ткань пародонта крыс с преднизолоновым пародонтитом: снижает активность кислой фосфатазы, увеличивает соотношение щелочная фосфатаза/кислая фосфатаза и степень минерализации, снижает степень атрофии альвеолярного отростка

6

SYNTHESIS AND APPLICATION OF CHITOSAN HYDROXYAPATITE: A REVIEW

51%

Vokhidova N. R., Ergashev K. H., Rashidova S. S.

Progress on Chemistry and Application of Chitin and its Derivatives

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2022

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vol. 27

5-34

EN