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  1. 1 Acta Biochimica Polonica

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  1. 1 1999

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  1. 1 Hołody D.

  2. 1 Strzeżek J.

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Heparin- and Zn^2+-binding proteins from boar seminal plasma

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Hołody D., Strzeżek J.
Acta Biochimica Polonica
|
1999
|
vol. 46
|
issue 4
935-939
EN
Low molecular mass, heparin-binding proteins from seminal plasma play an important role in gametes interaction whereas plasmatic Zn^{2+}-binding proteins stabilize chromatin and plasmalemma structures and protect spermatozoa in the female reproductive tract. By means of affinity chromatography the heparin- and Zn^{2+}-binding proteins were isolated from boar seminal plasma and both preparations were analyzed by reverse HPLC. Most of the proteins bound to heparine and Zn^{2+}-ions were classified as spermadhesins. Three fractions binding exclusively Zn^{2+} were isolated. They differ in amino-acid composition, content of glucosamine and content of protein components revealed by SDS/PAGE.
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