Search results

1

Assessment of biofilm formation by faecal strains of Enterococcus hirae from different species of animals

100%

Bino E., Lauková A., Kandričáková A., Nemcová R., Bino E., Lauková A., Kandričáková A., Nemcová R.

Polish Journal of Veterinary Sciences

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2018

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vol. 21

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issue 4

2

Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions

100%

Adamus-Białek W., Kubiak A., Czerwonka G.

|

2015

|

vol. 62

|

issue 4

765-771

EN

The ability to form different types of biofilm enables bacteria to survive in a harsh or toxic environment. Different structures of biofilms are related to different surfaces and environment of bacterial growth. The aim of this study was analysis of the biofilm formation of 115 clinical uropathogenic Escherichia coli strains under different growth conditions: surface for biofilm formation, medium composition and time of incubation. The biofilm formation after 24 h, 48 h, 72 h and 96 h was determined spectrophotometrically (A531) after crystal violet staining and it was correlated with bacterial growth (A600). The live and dead cells in biofilm structures was also observed on the glass surface by an epi-fluorescence microscope. Additionally, the presence of rpoS, sdiA and rscA genes was analyzed. The statistical significance was estimated by paired T-test. The observed biofilms were different for each particular strain. The biofilm formation was the highest in the rich medium (LB) after 24 h and its level hasn't changed in time. When biofilm level was compared to bacterial growth (relative biofilm) - it was higher in a minimal medium in comparison to enriched medium. These results suggest that most of the bacterial cells prefer to live in a biofilm community under the difficult environmental conditions. Moreover, biofilm formation on polyurethane surface did not correlate with biofilm formation on glass. It suggests that mechanisms of biofilm formation can be correlated with other bacterial properties. This phenomenon may explain different types of biofilm formation among one species and even one pathotype - uropathogenic Escherichia coli.

3

INFLUENCE OF SUBSTRATE LOADING AND DETACHMENT CONDITIONS ON A PLANE BIOFILM BEHAVIOUR

100%

Ledakowicz S., Blatkiewicz M., Tabiś B.

Chemical and Process Engineering

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2013

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issue 4

4

Opportunistic Gram-negative rods' capability of creating biofilm structures on polivynyl chloride and styrene-acronitrile copolymer surfaces

100%

Zabielska J., Kunicka-Styczyńska A., Rajkowska K., Tyfa A.

|

2015

|

vol. 62

|

issue 4

733-737

EN

Biofilms are highly organized microbial communities displaying high resistance to disinfectants and other external environmental factors. Medical equipment, such as stents and catheters, can be colonized by a variety of bacteria including opportunistic pathogens circulating in the environment and dangerous to immunocompromised patients. Application of materials resistant to biofilm formation will minimize the risk of patients' infection. Hence, the aim of this research was to determine the biofilm growth of environmental bacteria isolates on polyvinyl chloride and styrene-acronitrile copolymer surfaces. Nine strains (Pseudomonas aeruginosa, Burkholderia cepacia and Serratia liquefacies) isolated from cosmetics, and a reference P. aeruginosa strain ATCC 15442, were tested. The ability and dynamics of biofilm formation on intubation catheters (30°C, up to 24 h) in bacterial growth cultures (107-108 CFU/ml) was investigated, with subsequent sonication and quantification by agar plate count method. The results indicated that all the tested bacteria expressed a strong ability for the polymer surface adhesion, reaching 4.6 to 6.7 log CFU/cm2 after 30 minutes. Moreover, for the majority of strains, the level of 24-hour biofilm production was from 6.67-7.61 log CFU/cm2. This research indicates that the environmental strains circulating between the cosmetics and patients may pose a threat of biofilm formation on medical equipment surfaces, and presumably in the clinical surroundings as well.

5

Microscopic findings for the study of biofilms in food environments

100%

Olszewska M.

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2013

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vol. 60

|

issue 4

531-537

EN

The capability of bacteria to colonize food processing surfaces and to form biofilm has become an emerging concern for food industry. The presence and persistence of biofilm on food processing surfaces may pose a risk of food spoilage or food poisoning. A better understanding of bacterial adhesion and resistance of biofilms is needed to ensure quality and safety of food products. This review focuses on microscopic approaches incorporated to explore biofilm mode of existence in food processing environments. An application of antimicrobial agents for the biofilm control, in particular for bacteria connected with food processing environments, is also highlighted. In addition, some aspects of biofilm resistance, especially the phenomenon of persister cells, are discussed.

6

INFLUENCE OF SUBSTRATE LOADING AND DETACHMENT CONDITIONS ON A PLANE BIOFILM BEHAVIOUR

100%

Blatkiewicz M., Tabiś B., Ledakowicz S.

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vol. 34

|

issue 4

463-477

EN

A mathematical model of a plane, steady state biofilm, with the use of a single substrate kinetics, was proposed. A set of differential equations was solved. In order to analyse the biofilm’s behaviour, a number of simulations were performed. The simulations included varying process parameters such as detachment coefficient and substrate loading. Two detachment models were taken into consideration: one describing the detachment ratio as proportional to the thickness of the biofilm, and the other one proportional to the thickness of the biofilm squared. The results provided information about substrate and live cell distribution in biofilm and the influence of certain parameters on biofilm behaviour.

7

Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria

100%

Moryl M., Spętana M., Dziubek K., Paraszkiewicz K., Różalska S., Płaza G., Różalski A.

|

2015

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vol. 62

|

issue 4

725-732

EN

The aim of this study was to investigate the antimicrobial effect of lipopeptide biosurfactants from surfactin, iturin and fengycin families, synthesised by the Bacillus subtilis I'1a strain, on uropathogenic bacteria, including the effects on planktonic growth, processes of biofilm formation and dislodging. Antimicrobial activity was tested against 32 uropathogenic strains belonging to 12 different species of Gram-negative and Gram-positive bacteria. The sensitivity of 25 tested bacterial strains to the B. subtilis I'1a filtrate was confirmed by an agar diffusion assay. None of the strains seemed to be sensitive to pure surfactin at concentrations ranging from 0.1 mg × ml-1 to 0.4 mg ml-1. After the treatment of uropathogens with B. subtilis lipopeptides, the metabolic activity of planktonic cells was inhibited by 88.05±3.96% in the case of 21 studied uropathogens, the process of biofilm formation was reduced by 88.15±4.77% in the case of 24 uropathogens and mature biofilms of 18 strains were dislodged by about 81.20±4.72%. Ten strains of uropathogenic bacteria were selected to study the antimicrobial activity of surfactin (concentrations 0.1, 0.2 and 0.4 mg × ml-1). Surfactin had no influence on the metabolic activity of planktonic forms of uropathogens, however, biofilms of 5 tested strains were reduced by 64.77±9.05% in the presence of this biosurfactant at the concentration 0.1 mg × ml-1. The negative effect of the compound on the biofilm formation process was observed at all concentrations used. The above-described results were fully confirmed by CLSM. It could suggest that synergistic application of biosurfactants could be efficient in uropathogen eradication.

8

Silver nanoparticles as an alternative strategy against bacterial biofilms

100%

Markowska K., Grudniak A., Wolska K.

|

2013

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vol. 60

|

issue 4

523-530

EN

Biofilms are complex bacterial communities that resist the action of antibiotics and the human immune system. Bacteria within biofilms are the cause of numerous, almost impossible to eradicate, persistent infections. Biofilms can form on many medical devices and implants, and so have an enormous impact on medicine. Due to the lack of effective anti-biofilm antibiotics, novel alternative compounds or strategies are urgently required. This review describes some of the latest approaches in the field of biofilm treatment. New anti-biofilm technologies target different stages in the biofilm formation process. Some act to modify the colonized biomaterials to make them resistant to biofilm formation. One potentially important candidate treatment uses silver nanoparticles that show anti-bacterial and anti-biofilm activity. The biological action of nano-silver is complex and seems to involve a number of pathways. However, there have been few reports on the anti-biofilm activity of silver nanoparticles and the precise mechanism underlying their action remains unresolved. Here, we describe some anti-biofilm approaches employing AgNPs and consider the challenges and problems that need to be addressed in order to make silver nanoparticles a part of an effective anti-biofilm strategy.

9

Comparison of antibiotic resistance and virulence between biofilm-producing and non-producing clinical isolates of Enterococcus faecium

100%

Sieńko A., Wieczorek P., Majewski P., Ojdana D., Wieczorek A., Olszańska D., Tryniszewska E.

|

2015

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vol. 62

|

issue 4

859-866

EN

An increase in the antibiotic resistance among Enterococcus faecium strains has been observed worldwide. Moreover, this bacteria has the ability to produce several virulence factors and to form biofilm that plays an important role in human infections. This study was designed to compare the antibiotic resistance and the prevalence of genes encoding surface protein (esp), aggregation substance (as), surface adhesin (efaA), collagen adhesin (ace), gelatinase (gelE), and hialuronidase (hyl) between biofilm-producing and non-producing E. faecium strains. Therefore, ninety E. faecium clinical isolates were tested for biofilm-forming ability, and then were assigned to two groups: biofilm-positive (BIO+, n =70) and biofilm-negative (BIO-, n = 20). Comparison of these groups showed that BIO+ isolates were resistant to β-lactams, whereas 10% of BIO- strains were susceptible to ampicillin (statistically significant difference, p = 0.007) and 5% to imipenem. Linezolid and tigecycline were the only antibiotics active against all tested isolates. Analysis of the virulence factors revealed that ace, efaA, and gelE genes occurred more frequently in BIO- strains (ace in 50% BIO+ vs. 75% BIO-; efaA 44.3% vs. 85%; gelE 2.9% vs. 15%, respectively), while hyl gene appeared more frequently in BIO+ isolates (87.1% BIO+ vs. 65% BIO-). These differences were significant (p < 0.05). We concluded that BIO+ strains were more resistant to antibiotics than BIO- strains, but interestingly, BIO- isolates were characterized by possession of higher virulence capabilities.

10

Prospektywne badanie obserwacyjne biofilmu bakteryjnego na powierzchni migdałka gardłowego w populacji pediatrycznej i związane z tym implikacje kliniczne

88%

Subtil J., Bajanca-Lavado M. P., Rodrigues J., Duarte A., Reis L., Nogueira I., Jordao L.

Otolaryngologia Polska

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2019

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vol. 73(1)

22-28

PL

Wprowadzenie: Migdałek gardłowy jest tkanką limfoidalną nosowej części gardła, pełniącą istotną rolę w ochronie gospodarza przed zakażeniami górnych dróg oddechowych. Niemniej, migdałek gardłowy może jednocześnie stanowić rezerwuar drobnoustrojów, wywołujących infekcje górnych dróg oddechowych i zapalenia ucha środkowego, szczególnie u dzieci. Cel: Ocena i porównanie związku między gromadzeniem się biofilmu na migdałku gardłowym a częstością występowania nawracających zakażeń w populacji pediatrycznej poddanej adenoidektomii ze wskazań zakaźnych lub niezakaźnych. Metody: Do oceny biofilmu na powierzchni migdałków gardłowych zastosowano skaningową mikroskopię elektronową; gromadzenie się biofilmu in vitro monitorowano przy pomocy testu z fioletem krystalicznym; wrażliwość na antybiotyki oceniano zgodnie z wytycznymi EUCAST; typowanie otoczek H. influenzae przeprowadzono przy użyciu metody PCR. Wyniki: Biofilm był obecny w 27,4% próbek migdałków gardłowych i nie stwierdzono statystycznie istotnych różnic pomiędzy grupami zakaźnymi i niezakaźnymi. W warunkach in vitro bakterie najistotniejsze klinicznie, takie jak H. influenzae, S. aureus, S. pyogenes, S. pneumoniae i M. catarrhalis, brały udział w tworzeniu się biofilmu w stopniu umiarkowanym (71,7%). Łącznie 55,3% z wymienionych bakterii było średniowrażliwych / opornych na co najmniej jeden z badanych antybiotyków. Nie stwierdzono związku między zdolnością do tworzenia biofilmu in vitro a obecnością biofilmu na powierzchni migdałków gardłowych ani opornością na antybiotyki. Wszystkie szczepy H.influenzae zostały opisane jako nietypowalne (bezotoczkowe). Wniosek: Obecność biofilmu na powierzchni migdałków gardłowych była niezależna od pochodzenia próbki klinicznej. Zdolność bakterii do tworzenia biofilmu in vitro nie może być stosowana w przewidywaniu powstawania biofilmu in vivo. Brak zależności pomiędzy tworzeniem się biofilmu a wykrywaną opornością na antybiotyki przyczynia się do poddania w wątpliwość znaczenia biofilmu w patogenezie chorób zakaźnych.

11

Effect of nutrient and stress factors on polysaccharides synthesis in Proteus mirabilis biofilm

88%

Moryl M., Kaleta A., Strzelecki K., Różalska S., Różalski A.

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2014

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vol. 61

|

issue 1

133-139

EN

The extracellular matrix in biofilm consists of water, proteins, polysaccharides, nucleic acids and phospholipids. Synthesis of these components is influenced by many factors, e.g. environment conditions or carbon source. The aim of the study was to analyse polysaccharides levels in Proteus mirabilis biofilms after exposure to stress and nutritional conditions. Biofilms of 22 P. mirabilis strains were cultivated for 24, 48, 72 hours, 1 and 2 weeks in tryptone soya broth or in modified media containing an additional amount of nutrients (glucose, albumin) or stress factors (cefotaxime, pH 4, nutrient depletion). Proteins and total polysaccharides levels were studied by Lowry and the phenol-sulphuric acid methods, respectively. Glycoproteins levels were calculated by ELLA with the use of selected lectins (WGA and HPA). For CLSM analysis dual fluorescent staining was applied with SYTO 13 and WGA-TRITC. In optimal conditions the levels of polysaccharides were from 0 to 442 μg/mg of protein and differed depending on the strains and cultivation time. The agents used in this study had a significant impact on the polysaccharides synthesis in the P. mirabilis biofilm. Among all studied components (depending on tested methods), glucose and cefotaxime stimulated the greatest production of polysaccharides by P. mirabilis strains (more than a twofold increase). For most tested strains the highest amounts of sugars were detected after one week of incubation. CLSM analysis confirmed the overproduction of N-acetyloglucosamine in biofilms after cultivation in nutrient and stress conditions, with the level 111-1134%, which varied depending on the P. mirabilis strain and the test factor.

12

Życie w społeczności - warunki powstawania biofilmu

88%

Cłapa T., Selwet M., Narożna D.

Kosmos

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2016

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vol. 65

|

issue 3

463-468

PL

Wszystkie organizmy żywe podlegają wpływom innych organizmów wykazując różnego rodzaju zachowania społeczne. Mikroorganizmy nie są wyjątkiem. Komórki bakterii wolno żyjących (planktonicznych) są w stanie nie tylko wydzielać związki sygnałowe ale także mogą je odbierać. Proces komunikacji bakterii opierający się na tego typu sygnałach chemicznych jest szczególnie ważny w wielokomórkowych strukturach, jakie mogą tworzyć bakterie, czyli biofilmach. Takie społeczności bakteryjne są w stanie wzrastać w wielu środowiskach biotycznych jak i abiotycznych, niejednokrotnie w warunkach ekstremalnych. Proces komunikacji pomiędzy komórkami jest bardzo ważny, umożliwia nie tylko dzielenie się funkcjami fizjologiczno-metabolicznymi, ale również sprzyja ewolucji bakterii wskutek horyzontalnego transferu genów. Istotne jest poznanie nie tylko sposobu komunikacji pomiędzy mikroorganizmami, ale także warunków w jakich może zachodzić oraz procesów metabolicznych, którymi może ona sterować.

EN

All living organisms interact with each other and may exhibit cooperative behavior. Bacteria are not an exception. Free-living cells (planctonic cells) are able to communicate to each other by using specific types of chemical compounds. Such communication processes between bacterial cells are particularly important in multicellular structures, referred to as biofilms. Those structures are able to grow both in biotic and abiotic environments, in many cases even in very extreme conditions. The cell-communication processes are so important in bacterial biofilms for they provide sharing of physiological and metabolic functions between different species and thus stimulation of horizontal gene transfer that leads to bacterial evolution. Therefore, of importance is not only discovery and understanding of the communication system between microorganisms, but also of the conditions in which they may occur and influence cellular metabolic processes.

13

Analysis of ciprofloxacin and gentamicin diffusion in Proteus mirabilis O18 biofilm by laser interferometry method

88%

Arabski M., Wąsik S., Zych M., Łakomiec W., Kaca W.

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2013

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vol. 60

|

issue 4

707-711

EN

Laser interferometry is a measurement technique used in physical sciences, with a potential for new applications in microbiology. Our previously studies, focused on the quantitative analysis of antibiotics diffusion through membranes or their releasing from gel structure, indicate that this method might be useful in analysis of substances diffusion across the bacterial biofilms. As antibiotic - biofilm interaction model, we tested above method for determination of ciprofloxacin or gentamicin diffusion through Proteus mirabilis O18 biofilm. Laser interferometry analysis of antibiotics diffusion showed that the amount of ciprofloxacin transported through mature biofilm is 1.9 times higher than gentamicin. It was correlated with lower level of gentamicin in compare to the level of ciprofloxacin in biofilm, which amounts were predicted in biofilm during diffusion process by laser interferometry method. We suggest that the analysis of antibiotic diffusion in biofilm might by helpful in evaluation of effectiveness of antibacterial agents.

14

Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H Nuclear Magnetic Resonance search for candidate biomarkers

88%

Junka A., Deja S., Smutnicka D., Szymczyk P., Ziółkowski G., Bartoszewicz M., Młynarz P.

|

2013

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vol. 60

|

issue 4

701-706

EN

Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.

15

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

88%

Chabowski K., Junka A. F., Piasecki T., Nowak D., Nitsch K., Smutnicka D., Bartoszewicz M., Moczała M., Szymczyk P.

Metrology and Measurement Systems

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2017

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vol. 24

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issue 2

16

Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle

88%

Miletić M., Vuković D., Živanović I., Dakić I., Soldatović I., Maletić D., Lazović S., Malović G., Petrović Z., Puač N.

Open Physics

|

2014

|

vol. 12

|

issue 3

160-167

EN

In numerous recent papers plasma chemistry of non equilibrium plasma sources operating at atmospheric pressure has been linked to plasma medical effects including sterilization. In this paper we present a study of the effectiveness of an atmospheric pressure plasma source, known as plasma needle, in inhibition of the growth of biofilm produced by methicillin resistant Staphylococcus aureus (MRSA). Even at the lowest powers the biofilms formed by inoculi of MRSA of 104 and 105 CFU have been strongly affected by plasma and growth in biofilms was inhibited. The eradication of the already formed biofilm was not achieved and it is required to go to more effective sources.

17

rapid detection for the inhibition of phosphoglucose isomerase from Escherichia coli by mercury(II) chloride based on TLC-autographic analysis – preliminary studies

88%

Paradowska K., Lutek J., Ginalska G.

Current Issues in Pharmacy and Medical Sciences

|

2014

|

vol. 27

|

issue 2

127-130

EN

The quest for new techniques for screening inhibitors of phosphoglucose isomerase is crucially important owing to therapeutic control of chronic bacterial infections associated with the biosynthesis of bacterial biofilm. According to the new method, yellowish zones against the purple background could be visually observed where phosphoglucose isomerase activity was inhibited. The new protocol with NADPH/NBT/PMS staining for TLC-autographic method was able to detect PGI inhibition by pure reference substance as mercury(II) chloride.

18

MATHEMATICAL MODELLING AND STATIONARY CHARACTERISTICS OF A TWO-PHASE FLUIDISED-BED BIOREACTOR WITH EXTERNAL AERATION

88%

Tabiś B., Stryjewski W. S.

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vol. 34

|

issue 4

435-448

EN

A mathematical model for a two-phase fluidised bed bioreactor with liquid recirculation and an external aerator was proposed. A stationary nonlinear analysis of such a bioreactor for an aerobic process with double-substrate kinetics was carried out. The influences of a volumetric fraction of solid carriers in the liquid phase, the rate of active biomass transfer from the biofilm to the liquid, the concentration of carbonaceous substrate, the mean residence time of the liquid and the efficiency of the external aerator on the steady state characteristics of the bioreactor were described. A method for determination of the minimal recirculation ratio related to oxygen demand and fluidised bed conditions was presented. On the basis of the obtained results, it is possible to choose reasonable operating conditions of such plants and to determine constraints, while considering acceptable concentrations of a toxic substrate being degraded.

19

Evaluation of hydrophobicity and quantitative analysis of biofilm formation by Alicyclobacillus sp.

88%

Tyfa A., Kunicka-Styczyńska A., Zabielska J.

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2015

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vol. 62

|

issue 4

785-790

EN

Alicyclobacillus sp. are acidothermophilic bacteria frequently contaminating fruit based products (juices and juice concentrates). These sporulating bacteria are able to survive at elevated temperatures and highly acidic environments which causes difficulties in their removal from industrial environments. Although numerous literature data examine Alicyclobacillus sp. presence in fruit based products and methods of their elimination, there is still a limited knowledge on ability of these bacteria to adhere to abiotic surfaces. Therefore, the objective of this study was to determine Alicyclobacillus sp. cells' hydrophobicity and capability of biofilm formation on a glass surface. The degree of cells hydrophobicity, according to Microbial Adhesion to Hydrocarbon (MATH) and Salt Aggregation Test (SAT), was investigated for eleven environmental isolates from natural Polish habitats, identified as Alicyclobacillus sp., and a Alicyclobacillus acidoterrestris DSM 3922 reference strain. The dynamics of biofilm formation within 3-day incubation on a glass surface was evaluated and quantified by a plate count method both, for cultures with and without agitation. All of the bacterial strains tested expressed ability to colonize a glass surface and four environmental isolates were classified as fast-adherent strains. The mature biofilm structures were predominantly formed after 48 hours of incubation. Dynamic culturing conditions were observed to accelerate the biofilm formation. The majority of strains expressed a moderate hydrophobicity level both, in SAT (41.7%) and MATH-PBS (75.0%), as well as MATH-PUM (91.7%) tests. However, no correlation between hydrophobicity and cell adherence to a glass slide surface was observed.

20

Selective arylation of phenol proteted propygyl bromide via pd-catalysed Suzuki coupling reaction: synthesis, mechanistic studies by DFT calculations and Their Pharmacological Aspects"

75%

farooq h., rasool n., Ansari M. T., rizwan k., mahmood t., israr h., Ayub K., rasheed t., Zareen S., Akhtar m. N., iqbal s.

Acta Poloniae Pharmaceutica - Drug Research

|

2018

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vol. 75

|

issue 4

911-919

EN

Biaryls are the potential source of synthetic drugs. The present study describes the synthesis of a series of functionalized biphenyl derivatives (3a-3g) using Pd-catalyzed Suzuki coupling reaction. The experimental results revealed the facile synthesis of biphenyl derivatives (3a-3g) with notably high yield (80-88 %). Density functional theory (DFT) studies were performed by Gaussian 09 software in order to rationalize the selectivity of coupling at C-Br bond instead of C-Cl bond. In addition of synthesis, the biological activities (biofilm inhibition, hemolytic and anti-thrombolytic) of these novel compounds were investigated. These results exhibited good biofilm inhibition (5.86-65.8 %), hemolytic (1.32-30.1 %) and anti-thrombolytic activities (9.64-42.5 %), indicating the potential use of these compounds for pharmaceutical applications.

Refine search results

Assessment of biofilm formation by faecal strains of Enterococcus hirae from different species of animals

Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions

INFLUENCE OF SUBSTRATE LOADING AND DETACHMENT CONDITIONS ON A PLANE BIOFILM BEHAVIOUR

Opportunistic Gram-negative rods' capability of creating biofilm structures on polivynyl chloride and styrene-acronitrile copolymer surfaces

Microscopic findings for the study of biofilms in food environments

INFLUENCE OF SUBSTRATE LOADING AND DETACHMENT CONDITIONS ON A PLANE BIOFILM BEHAVIOUR

Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria

Silver nanoparticles as an alternative strategy against bacterial biofilms

Comparison of antibiotic resistance and virulence between biofilm-producing and non-producing clinical isolates of Enterococcus faecium

Prospektywne badanie obserwacyjne biofilmu bakteryjnego na powierzchni migdałka gardłowego w populacji pediatrycznej i związane z tym implikacje kliniczne

Effect of nutrient and stress factors on polysaccharides synthesis in Proteus mirabilis biofilm

Życie w społeczności - warunki powstawania biofilmu

Analysis of ciprofloxacin and gentamicin diffusion in Proteus mirabilis O18 biofilm by laser interferometry method

Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H Nuclear Magnetic Resonance search for candidate biomarkers

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle

rapid detection for the inhibition of phosphoglucose isomerase from Escherichia coli by mercury(II) chloride based on TLC-autographic analysis – preliminary studies

MATHEMATICAL MODELLING AND STATIONARY CHARACTERISTICS OF A TWO-PHASE FLUIDISED-BED BIOREACTOR WITH EXTERNAL AERATION

Evaluation of hydrophobicity and quantitative analysis of biofilm formation by Alicyclobacillus sp.

Selective arylation of phenol proteted propygyl bromide via pd-catalysed Suzuki coupling reaction: synthesis, mechanistic studies by DFT calculations and Their Pharmacological Aspects"