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EN
Hydrogel films composed of chitosan and agar or chitosan, agar and honey/bee pollen were prepared; they could be used as active wound dressings. During this study, various types of gel films were prepared using chitosan with different molecular weights and ratios of the two biopolymers. In addition, compositions with different amounts of honey or bee pollen were obtained. The selected samples were characterized by determining typical properties that are important for wound dressings. The best twocomponent films had a chitosan to agar weight ratio of 2:1. From among the examined tri-component films, the 2:1:0.5 weight ratio of chitosan, agar and 50 wt% honey solution was the best composition.
EN
In the paper, the adsorption of Acid Red 18 (AR18) on chitosan (CHs), sodium carboxymethyl cellulose (CMC) and agar (AGA) was researched. The adsorption capability of biosorbents was examined as a function of initial pH, time of contact and influence of initial concentration of dye. The adsorption kinetics was compared with the pseudo 1. and 2. order models. It was found that the dye adsorption occurred in accordance with the pseudo 2. order model. The experimental data of adsorption in the equilibrium state was analysed with the use of isotherms of the Freundlich, Langmuir and double-Langmuir models. It was found that for the description of dye adsorption on adsorbents, the double-Langmuir model was suitable, which was demonstrated by the determined values of the average relative error (ARE). The highest adsorption capacity and affinity to AR 18 was obtained for CHs, at 81.7 mg/g d.w and 0.997 L/mg, respectively. The experimental results show that CHs seems to be a promising biosorbent for AR 18 dye removal from aqueous solutions.
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2013
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vol. 60
|
issue 3
387-393
EN
An organic solvent and surfactant stable α-amylase was obtained from soybean seeds. The direct and indirect effect of various organic solvents (non-polar, polar protic, and polar aprotic) and surfactants on the activity and stability of free enzyme was determined. The enzyme showed a very high catalytic efficiency and stabilization against most of the organic solvents and surfactants tested, except for few. Those organic solvents and surfactants (like chloroform, dimethyl formamide, n-butanol, and Tween 20), which caused an inhibition in enzyme activity, were used to study their effects on immobilized enzyme. The inhibitory effect was found to be decreased in immobilized enzyme as compared to free enzyme indicating that immobilization imparted stability to the enzyme. Moreover, the possibility of reuse of the enzyme in the presence of the organic solvents and surfactants was increased upon immobilization. The stability of soybean α-amylase towards organic solvents and surfactants shows that it is a potential candidate for use in organic-solvent biocatalysis as well as in detergent industries.
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