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1

Influence of DNA concentration on the efficiency of bovine blastocyst production after WAP-bGH microinjection into germinal vesicle of immature oocytes, zygotes and embryos obtained in vivo or in vitro

100%
Jura J., Smorag Z., Katska L., Skrzyszowska M., Houdebine L.-M., Rynska B.
Biotechnologia
|
1997
|
issue 4
71-81
EN
Microinjection is one of the most successfully used methods to produce transgenic farm animals. But the effectiveness of transgenesis with the use of microinjection, specially in cattle is still low. Many steps of the transgenesis has been found to influence its effectiveness; DNA purity, the site of its injection, the culture system. There are no reports on the influence of a DNA vector concentration influence on the developmental ability of transformed bovine eggs to the blastocyst stage in the in vitro culture. In presented experiments we investigated the influence of different DNA concentrations on the developmental rate of microinjected immature bovine oocytes, zygotes and 2-cell embryos to the blastocyst stage.
2

Transgenic pigs: production and genetic modification

86%
Smorag Z., Jura J., Kopchick J. J., Gajda B., Skrzyszowska M., Rozycki M., Pasieka J.
Biotechnologia
|
1998
|
issue 2
145-152
EN
This paper presents the methods of transgenic pigs production and the results based on the long experience of the authors in this area. Moreover, the trends and current issues of transgenic modification in pigs are discussed.
3

Factors affecting production of transgenic farm animals: cattle and rabbits

86%
Jura J., Smorag Z., Katska L., Skrzyszowska M., Gajda B., Rynska B.
Biotechnologia
|
1998
|
issue 2
101-109
EN
There are many factors affecting transgenic farm animals production. One of them is the effectiveness of the transfer of zygotes and embryos obtained after DNA microinjection. Low effectiveness of the transfer of potentially transgenic blastocysts in cattle is due to their decreased developmental potential in comparison to the blastocysts developed from not microinjected zygotes. A simple short term in vitro culture used for rabbit zygotes after microinjection increased twice the number of produced potentially transgenic rabbits.
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