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Studies were carried out to investigate cultivation of some indigenous edible mushrooms in Anambra State. The most suitable substrates for cultivation of Pleurotus tuberregium and Volvariella volvacea were also investigated. Spawn of V. volvacea was obtained from the Department of Life Science, University of Benin, Edo State and sclerotia of P. Tuberregium purchased from Eke Awka Market, Anambra State were used for this study. Plantain leaves and oil palm bunch wastes were substrates employed for V. volvacea whereas topsoil was used for P. tuberregium cultivation. The methods used were site preparation, substrate preparation, soil preparation, sclerotia preparation, cultivation of sclerotia, irrigation, spawn cultivation and harvesting of mushrooms. Means were analyzed statistically using (ANOVA) to test for significance. Means were separated using Duncan’s Multiple Range Tests (DMRT).The highest number of fruit bodies of V. volvacea, 312.68 was obtained from plantain leaves on day 2 after primordial emergence whereas the least, 21.00 was recorded from oil palm bunch wastes on days 6 and 14, respectively. The highest number of P. tuberregium, 61.58 was recorded on day 12 after primordial emergence while the least, 20.49 was recorded on days 7, 13 and 14, respectively. All the three substrates utilized for cultivation of mushrooms in this study supported their growth and development. Plantain leaves supported fast colonization and produced high yield fruit bodies of V. volvacea. The ability to use agricultural wastes for cultivation of mushrooms will boost food production for ever increasing population.
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