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EN
The aim of the present study was to validate and implement a nutrition questionnaire to measure nutrition knowledge of university students, and athletes. Male, and female students (N = 476) voluntarily participated in the survey in 2019. Non-health related (n = 156), nutrition students (n = 163), and varsity athletes (n = 157) responded to the questionnaire, twice with 15 days’ interval between. In this study, new “General and Sport Nutrition Knowledge” (GSNK) and, two other "Short General Nutrition Knowledge” (SGNK) and, “Short Sport Nutrition Knowledge" (SSNK) questionnaires were used to determine the validation of the survey. One-way ANOVA, t Test, Pearson correlation coefficient, and Cronbach’s α statistics were used to evaluate validity and reliability (p < .05). The Internal consistency, test-retest reliability, concurrent validity with two similar tools, and construct validity among the groups of students for nutrition knowledge were employed throughout the data analysis. Nutrition students outperformed in all nutrition knowledge sections. Respectively, athletes were the second, and non-nutrition related students were the third in sport nutrition (p < .05). With regard to general and total nutrition knowledge scores, female students performed significantly better than males (p < .01). Modified questionnaire was found to be valid, reliable, and suitable tool for Eurasian university students, and athletes. Results also illustrated that the intermittently nutrition educations are required for athletes, as it is recommended in related literature.
Open Chemistry
|
2009
|
vol. 7
|
issue 3
354-361
EN
A quick method of quantitative determination of sulbactam in human plasma, using liquid chromatography-UV spectroscopy, has been developed and validated. After derivatization with imidazole, plasma samples were treated by direct deproteinization with acetonitrile as an extraction solvent. After ultracentrifugation, sulbactam extract was directly injected onto the LC column. Chromatographic separation was performed on TSK Gel Super ODS (50 mm × 4.6 mm i.d., 2 μm) using methanol and phosphate buffer with tetrabutylammonium hydroxide solution as a mobile phase. Gradient elution was employed. The method was fully validated according to the United States Food and Drug Administration requirements (linearity, precision, trueness, quantification limit, detection limit, recovery, specificity and stability). The calibration curves were linear within the concentration range of 0.05–4.0 μg mL−1. Good method/system precision and accuracy of the method were demonstrated. [...]
Open Chemistry
|
2013
|
vol. 11
|
issue 2
228-247
EN
A critical comparison was made of seven solid-phase extraction (SPE) sorbents for the fractionation and isolation of 21 important free and glycosidically-bound varietal volatile aroma compounds. The sample was subjected to SPE and the free aromatics were eluted with dichloromethane followed by elution of the glucoconjugates with methanol; after fractionation, the free fraction was analyzed directly by GC-MS while the sugar-bound fraction was enzymatically hydrolyzed to liberate the free compounds before analysis by GC-MS. The extraction efficiency for the free compounds was evaluated based on the analytes’ signal recovery and for the glycosidically-bound compounds in terms of the relative peak areas. The best results for both the free and bound fractions were obtained with the Isolute ENV+ resin. Following selection of the most efficient SPE material, a GC-MS method was validated (in terms of selectivity, linearity, limits of detection (LODs) and limits of quantification (LOQs), recovery, repeatability, within-laboratory reproducibility and uncertainty) for the quantitative determination of the free primary volatiles in white wines. Validation results are presented at 4 fortification levels (10, 50, 200 and 500 µL−1). Regarding linearity, the correlation coefficient of the matrix-matched calibration plots was ≥0.99 for all the compounds. The LOQs were in the range 0.6–17.5 µg L−1. Recoveries ranged from 61% to 120% while the% relative standard deviation of the within-laboratory reproducibility was in the range 1.3% to 17.7%. Finally, the% expanded uncertainty ranged from 3.1% to 40.3%. The method has been successfully applied to the analysis of 20 white wine samples. [...]
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