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Postnatal development of the rat striatum - a study using in situ DNA end labeling technique

100%

Maciejewska B., Lipowska M., Kowianski P., Domaradzka-Pytel B., Morys J.

vol. 58

issue 1

23-28

We have examined the development of rat striatum for evidence of cells dying in the process of physiological cell death. In present study we have indicated apoptotic cells in sections stained with cresyl violet (cell death characterized by pyknosis) or with DNA end labeling assay (TUNEL method). Our results demonstrated that cell loss during maturation of the rat striatum had the characteristics of apoptosis rather than necrosis. The greatest number of TUNEL - positive and pyknotic cells in the striatum were found during the first postnatal days; after 7th day of postnatal life a rapid decrease of its number was observed. After the second postnatal week no TUNEL-positive cells were observed in the striatum. Our analysis suggests that apoptotic cell death occurring during the development of striatal neuronal population takes place during the first week of postnatal life.

Apoptosis in relation to neuronal loss in experimental Creutzfeldt-Jakob disease in mice

88%

Jesionek-Kupnicka D., Kordek R., Buczynski J., Liberski P. P.

Acta Neurobiologiae Experimentalis

2001

vol. 61

issue 1

13-19

Apoptosis constitutes a genetically determined process to eliminate superfluous or damaged cells in tissues. Deficiencies in apoptosis regulation are involved in different pathologies including prion diseases. Some experimental studies show that neuronal loss - one of the hallmarks of prion diseases may be accomplished by apoptosis. We evaluated twenty five mice infected experimentally with the Fujisaki strains of CJD and sacrified sequentially in one week intervals. Apoptotic cells in various brain regions were detected by in situ end labelling (TUNEL) and electron microscopy in comparison with neuronal cell loss. The number of labelled cells per brain was very low - from a few labelled cells 6 weeks after inoculation to a maximum of 14 in the terminal stage. The number of neurones counted in 8 selected areas were considerably lower in terminally sick animals (20 and 21 week of incubation period) than in control mice. The mean value of loss of neuronal cells was 32%. The greatest loss (55%) of neurones was noted in the septal nuclei of the paraterminal body and the least lost (16%) in the hypothalamus. Compared to the extensive neuronal loss (30-50%), the number of apoptotic cells detected by in situ end labelling seems to be very low, and the process of neuronal death become more intensive during the progression of the disease.

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2 Acta Neurobiologiae Experimentalis

1 2001

1 1998

1 Buczynski J.

1 Domaradzka-Pytel B.

1 Jesionek-Kupnicka D.

1 Kordek R.

1 Kowianski P.

1 Liberski P. P.

1 Lipowska M.

1 Maciejewska B.

1 Morys J.

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Postnatal development of the rat striatum - a study using in situ DNA end labeling technique

Apoptosis in relation to neuronal loss in experimental Creutzfeldt-Jakob disease in mice