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1

EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections

100%
Wang H.-Y., Wei Y.-M., Yan Z.-H., Zheng Y.-L.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 3
35-42
EN
SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which trinucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.
2

EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections

100%
Wang H.-Y., Wei Y.-M., Yan Z.-H., Zheng Y.-L.
|
EN
SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which tri-nucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.
3

Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf. with reference to wheat breeding

70%
Wang H.-Y., Liu D.-C., Yan Z.-H., Wei Y.-M., Zheng Y.-L.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 4
365-369
EN
Cytological and agronomic characteristics of a F2 population from Triticum aestivum L. ? T. durum Desf. hybrids were analyzed plant by plant. Means of morphologic traits in the F2 population were similar to those of the low-value parent. On average, F2 hybrids had 36.54 chromosomes per plant, indicating that each gamete lost 2.73 chromosomes at meiosis of the F1 generation. More than half of plants had 36?39 chromosomes, so male gametes with 19?21 chromosomes seemed to be superior to the others. The distribution frequency of chromosomes in this study differed from that in a previous report, where a different tetraploid wheat was used. This shows that a different breeding strategy may need to be taken when exploiting a different tetraploid wheat. According to our results, some plants with 42 chromosomes, having all the wheat A, B and D chromosomes, would appear in the F3 population, which provides a chance to obtain stable bread wheat lines from the self-pollinated progenies. Alternatively, the desirable individuals of the F2 population were backcrossed to bread wheat, which is very useful and efficient for the improvement of bread wheat by exploiting desirable genes in durum wheat.
4

Microsatellite mapping of the genes for brittle rachis on homoeologous group 3 chromosomes in tetraploid and hexaploid wheats

70%
Watanabe n., Fujii Y., Kato N., Ban T., Martinek P.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 2
93-98
EN
The brittle rachis character, which causes spontaneous shattering of spikelets, has an adaptive value in wild grass species. The loci Br1 and Br2 in durum wheat (Triticum durum Desf.) and Br3 in hexaploid wheat (T. aestivum L.) determine disarticulation of rachides above the junction of the rachilla with the rachis such that a fragment of rachis is attached below each spikelet. Using microsatellite markers, the loci Br1, Br2 and Br3 were mapped on the homoeologous group 3 chromosomes. The Br2 locus was located on the short arm of chromosome 3A and linked with the centromeric marker, Xgwm32, at a distance of 13.3 cM. The Br3 locus was located on the short arm of chromosome 3B and linked with the centromeric marker, Xgwm72 (at a distance of 14.2 cM). The Br1 locus was located on the short arm of chromosome 3D. The distance of Br1 from the centromeric marker Xgdm72 was 25.3 cM. Mapping the Br1, Br2 and Br3 loci of the brittle rachis suggests the homoeologous origin of these 3 loci for brittle rachides. Since the genes for brittle rachis have been retained in the gene pool of durum wheat, the more closely linked markers with the brittle rachis locus are required to select against brittle rachis genotypes and then to avoid yield loss in improved cultivars.
5

Gene transfer from Aegilops ventricosa to Triticum aestivum

61%
Tyrka M., Stefanowska G., Brzezinski W.
Biotechnologia
|
2001
|
issue 2
57-62
EN
RAPD (Randomly Amplified Polymorphic DNA) and glutenin SDS-PAGE analyses were performed on wheat hybrid strains derived from multiple crosses of hybrids between Ae. ventricosa and T. durum with hexaploid wheat. We found Aegilops specific bands (G03580 and T02990) in two hybrid strains (VGPB and VGPAA) using RAPD method. The presence of Ae. ventricosa specific fraction of glutenin was shown in one wheat hybrid strain (VGPP).
6

Salt tolerance of Triticum monococcum L., T. dicoccum (Schrank) Schubl., T. durum Desf. and T. aestivum L. seedlings

61%
Prazak R.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 3
289-292
EN
Responses to salt stress of diploid wheat Triticum monococcum L., tetraploid wheats T. dicoccum (Schrank) Schubl. and T. durum Desf. cv. Grandur, and hexaploid wheats T. aestivum L. cvs. Begra and Gama were studied. Seeds were germinated on filter paper in Petri dishes, moistened in Hoagland medium containing 0 (control), 10, 20, 50, 100 and 200 mM NaCl. Index of tolerance to salt treatments was calculated on the basis of shoot length. Increase in salt concentration led to decrease in seed germination and inhibition of shoot and root elongation. Hexaploid and diploid wheats were more tolerant to salt stress than tetraploid wheats.
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