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Three dipoloid Trifolium (Fabaceae) species, T.campestre, T.fragiferum and T.montanum, were electrophoretically assyed to elucidate the range anf organization of genetic variation in relation to the mating system, reproduction mode and longevity of the species.T.campestre, is an annual self-pollinated species, T.fragiferum and T.montanum are cross-pollinated perennials.The former species reproduces both sexually and vegetatively, whereas the latter solely on sexual reproduction.Several populations of each species collected throughoutnPoland were surveyed for 15 enzymes.The measures of variation included:proportion of polymorphic loci per population, average number of alleles per locus, average gene diversity (He) and genetic distance (D).To describe the organization of diversity in each species, total genetic variation per locus (Ht) was calculated.Both cross-polinated species had more polymorphic loci, higher numbers of alleles per locus and higher level of diversity than self-pollinated T.campestre.Most of genetic variation of the latter species was allocated among polulation unlike in T.fragiferum and T.montanum.
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