Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 9

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  TRANSGENIC ANIMAL
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Sperm cells as DNA vectors for production of transgenic animals

100%
Jurkiewicz J.
Biotechnologia
|
2006
|
issue 1
29-43
EN
The transgenic animals are obtained mainly by microinjection of exogenous DNA into male pronuclei of the zygote. The efficiency of this method is not satisfactory, especially in domestic animals. Using the sperm cells as vectors for transfering exogenous DNA sequences into the oocyte during the process of fertilization seems to be an easy and simple method for production of transgenic animals. It was discovered that sperm cells of all species show the spontaneous ability to take up exogenous DNA. Exogenous DNA binding is not accidental, it always occours in the postacrosomal segment of the sperm head. The interaction is ionic, reversible, sequence ? independent, and not restricted to DNA, but can be reproduced using other negetively charged macromolecules. The introduction of DNA into sperm cells is possible using different methods, however, any of these methods could be considered as a routine one. The mechanisms of taking up and associationig DNA by sperm are being constantly revealed and better understood.
2

Heart of transgenic pigs as a substitute of human heart. Expectations and reality

100%
Grzybowski G., Lubieniecka J., Kantoch M. J.
Biotechnologia
|
1998
|
issue 3
21-34
EN
The authors discuss hopes and fears, expectations and reality of xenotransplantations.
3

Reproductive performance of a transgenic boar carrying the bovine growth hormone gene (bGH)

80%
Rejduch B., Slota E., Sysa P., Rozycki M.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 3
337-341
EN
The aim of this study was to obtain information on the possible influence of the bovine growth hormone gene (bGH) on gametogenesis and reproductive parameters of a 2-year-old Polish Landrace transgenic boar. The bGH gene construct had been introduced into the zygote of the boar with the use of the microinjection technique. On the basis of the available documentation we established that the fertility of the investigated transgenic (bGH) boar was low in comparison with other animals of the Polish Landrace breed, with a poor libido, ineffective matings and, on average, 3 live piglets less per litter. Samples of testis tissue from the boar were obtained after castration. In total, we observed 100 spermatocytes and all of them had normally paired bivalents. It is possible that the boar?s lower fertility was caused by some, as yet unknown factor.
4

Animals as bioreactors ? the future of pharmaceutical industry?

80%
Tyczewska A., Bakowska-Zywicka K.
Biotechnologia
|
2008
|
issue 3
64-70
EN
Biotechnology products and its application raise many controversies. Discussions are carried out where the supporters of GMO are underlining the qualities of genetically modified organisms, and sceptics are pointing the dangers that, in their perspective, are exceeding the benefits. In this article, we intend to show the qualities resulting from the use of transgenic animals to produce cheaper drugs, as biotechnology and genetic engineering methods gave the possibility to use animals as bioreactors.
5

The organ transplantation ? a challenge for biotechnology

80%
Jasinski A., Slomski R., Szalata M., Lipinski D.
Biotechnologia
|
2006
|
issue 1
7-28
EN
The use of animals as a source of organs and tissues for xenotransplantation can overcome the growing shortage of human organ donors. However, xenoreactive antibodies present in humans directed against swine Gal antigen on the surface of xenograft donor cells leads to the complement activation and immediate xenograft rejection as a consequence of hyperacute immunological reaction. The graft of genetically modified organ of a swine would be tolerated with simultaneous administration of medicines decreasing other less severe immunological reactions. This review summarizes the clinical history and rationale for xenotransplantation, recent progress in understanding the physiologic, immunologic, and infectious obstacles to xenotransplantation and some of the strategies being pursued to overcome these dificulties.
6

Transgenic animals in agriculture and medicine

80%
Rosochacki S. J., Zwierzchowski L.
Biotechnologia
|
1999
|
issue 2
7-24
EN
One of the most important issues in the use of transgenic technology in animal breeding is production of meat. For that purpose growth enhancing DNA sequences are introduced to the genome of pigs, sheep, cattle, rabbits and fish. The structural elements of the introduced genes usually GH, GRF or IGF-I, which are combined with the regulatory elements. In the case of transgenic swine, the higher growth rate, and the lower fat content were achieved by using these gene constructs. However, a number of such swines were sterile and had some other pathological problems. Similar and even more serious problems, were noticed in the case of transgenic sheep up. To date, most transgenic livestock projects focused on enhancing growth in farm animals by overexpressing growth hormone have to pathological changes in transgenic farm animals; only in transgenic fish no such problems have been ecountered. Fast growing transgenic trouts, carps and salmons carrying the so called 'all fish gene constructs' could be used even right now as food. Another goal in transgenic livestock projects is manufacturing of biologicaly active human proteins in the mammary gland. Transgenic sheep, goats, pigs and cows which produce human pharmaceuticals in their milk have been obtained. Some of such proteins undergo clinical trials. In another arena, it is planned to modify the milk of ruminants in order to obtain better product, which will be used in the dairy industry. It is possible to introduce more copies of milk protein genes into the genome, 'improving' the existing genes, or to inhibit the expression of some genes - thus reducing or eliminating the production of unwanted proteins. Another way to modify milk composition is the so called 'humanisation' of cow's milk by changing the proportion of cow's to human proteins. So far, all these modifications in the area of milk proteins are done mostly on laboratory animals and the introduction of these possibilities to farm animals would be desirable. Except for fish, transgenic farm animals are relatively safe to the environment; the probability of transgenic animals surviving and reproducing out of farms is rather very low. On the contrary, introduction of highly productive farm animals (cows, pigs) to breeding would be profitable to the environment and the called 'transgenic bioreactors' would also cause decrease of chemical or pharmaceutical industry contamination to the environment. For consumers, the products obtained from transgenic animals should be safe. 'Transgenic' products, which normaly exist in the nature, are not more unsafe than their natural counterparts. There is a clear consumer demand for testing all new products which normaly do not exist in the nature, i.e. proteins with totaly new amino acid sequence, before their introducing into the market. However, this is also valid for all novel food products, not only those obtained by biotechnology.
7

Production of therapeutic proteins in the semen of transgenic animals

80%
Lipinski D., Juzwa W., Zeyland J., Slomski R.
Biotechnologia
|
2006
|
issue 1
44-52
EN
The expression of the recombinant proteins by transgenic animals represents an opportunity to achieve cost-effective, large-scale production of a wide variety of therapeutics. Among transgenic animal production systems, the transgenic mammary gland is the most advanced. However, the production of proteins in milk is limited by a relatively long interval from birth to first lactation encountered with domestic livestock, the discontinuous nature of the lactation cycle and the substantial time and material investments required to produce transgenic dairy animals. The semen of transgenic boar represents an alternative platform for the production of therapeutic proteins. The expression of such proteins in the male accessory glands, particularly in the seminal vesicle epithelium can be controlled by gene regulatory sequences specific to these tissues. In this review, we consider the possibility of using such regulatory sequences to drive the production of foreign proteins into the seminal fluids of transgenic animals. Application of this technology to pigs which can be ejaculated 2-3 times per week (200-300 ml per ejaculate), could lead to the annual production of several grams of recombinant protein.
8

Genetic gain in livestock populations and application of modern biotechniques of gametes and embryons

80%
Szwaczkowski T., Switonski M.
Biotechnologia
|
1993
|
issue 2
46-54
EN
The objective of this paper was to present an influence of some new biotechnologies on the increase of the livestock genetic gain. The effects of the following biotechniques were discussed: embryo transfer, multiple ovulation, in vitro fertilization of oocytes, embryo and semen sexing,cloning as well as creating transgenic animals. Application of these biotechniques increases selection intensity and descreases genetic variability (except for creating transgenic individuals). Moreover, modification of classical models of evaluation of the genetic value of animals is necessary.
9

Biotechnolgical methods implemented for improvement of farm animals as a food source with increased unsaturated fatty acids

80%
Ostaszewska I., Sablik P.
Biotechnologia
|
2008
|
issue 3
153-158
EN
Transgenic animals became easy and open for general use as a source of recombinant proteins and essential components like unsaturated fatty acids. That possibility can completely change the life and health of humans. Thanks to genetic engineering, it is possible to increase the level of healthy fatty acids in animal organism, with the help of e.g. stearoyl-CoA desaturase, fat-1 gene. Milk with changed fatty acids composition could be a cure for people with obesity and vascular problems, as also can be an alternative source of long chain polyunsaturated fatty acids for oil-rich fish and fish liver oils. What is more, also natural environment can be saved by those animals.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.