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  1. 2 Biotechnologia

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  1. 1 2002

  2. 1 1999

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  1. 2 Czarnecka-Verner E.

  2. 2 Gurley W. B.

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1

Plant heat shock transcription factors: divergence in structure and function

100%
Czarnecka-Verner E., Gurley W. B.
Biotechnologia
|
1999
|
issue 3
125-142
EN
A multitude of heat shock transcription factors (HSFs) have been isolated and characterized from various plant species (17-23). Based on a phylogeny analysis of the DNA binding domains and organization of oligomerization domains, they have been assigned to class A and B of the plant HSF family (20,24 and this paper). None of the tested soybean or Arabidopsis HSF class B members were able to function as transcriptional activators and are, therefore, considered to be inert (26,59). Conversely, class A HSFs from tomato and Arabidopsis displayed an intrinsic transcriptional activation potential (26,50). There seems to be variation among plant class A HSFs regarding their transcriptional activation functions: some play a key role in activation of the heat shock response, while others act in an auxiliary capacity as HSF activity boosters (54). In contrast, the class B inert HSFs are able to trans-attenuate the transcriptional activity of activator HSFs (26). We postulated that heat shock regulation in plants may differ from metazoans by partitioning negative and positive functional domains onto separate HSF proteins (59). In plants two classes of HSFs exist: class A members which function as activators of HSP gene expression, and a novel class B (inert HSFs) which is largely specialized for repression, or attenuation, of the heat shock response.
2

Arabidopsis class A and B HSFs show a spectrum of transcriptional activity

88%
Czarnecka-Verner E., Gurley W. B.
Biotechnologia
|
2002
|
issue 3
39-52
EN
A plethora of heat shock transcription factors (HSFs) has been obtained from various plant species (33,45-48,50,51). The Arabidopsis genome sequencing project provided confirmation of the existence of at least twenty one HSFs which were classified into three major classes, A, B and C, and numerous subclasses (9). Members of HSF class A displayed differential transcriptional activities in tobacco protoplasts that varied from 15- to 50-fold above the control level. This diversity of activity levels may reflect HSF variations regarding their transcriptional activation functions- some of the members might be the major heat inducible HSFs (class A1 HSFs), while others act in an auxiliary capacity as HSF activity boosters (38). Two new class B HSFs showed no transcriptional activation potential; however, they differed significantly in their ability to bind to heat shock elements (HSEs). The efficiency in HSE binding was linked directly with the ability to suppress the activity of endogenous tobacco HSFs. The suppression of endogenous HSFs by class B members provides further evidence that class B HSFs are not transcriptional activators, but are able to trans-attenuate the transcriptional activity of bona fide activator HSFs (34,41). The transcriptional competency of class C HSFs has not been determined.
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