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  1. 1 Folia Biologica

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  1. 1 1995

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  1. 1 Woszczynski M.

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Third genetic polymorphism of tear proteins Rtp-3 in rat

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Woszczynski M.
Folia Biologica
|
1995
|
vol. 43
|
issue 1-2
69-73
EN
Two genetic markers, tear proteins RTP-1 and RTP-2, were studied in six inbred rat strains by the agarose gel electrophoretic method.An RTP-2A fast migration band was found in M520/W, AUG/W, and BN/HanW strain whereas in WAG/W, L-E/W and SPRD/W rats no RTP-2 band was detectable.In the case of RTP-1 there always occured only one band with a different migration speed in gel according to the representing allele.The slow band RTP-1B was observed in rat strains WAG/W, AUG/W, SPRD/W, L-E/W, and M520/W, the fast band RTP-1A being observed only in BN/HanW rats.Moreover, a considerable sex difference in the expression of tear protein in all the tested strains was observed.The expression of a third independent rat tear protein locus whose product, controlled by androgens, was found to be present only in females and castrated males.It is proposed to designate it Rtp-3 with three alleles.The discrepancy of these results with the observation by Kondo et al. concerning Rtp-1 alleles has been discussed.
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