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EN
Bioremediation of soil contaminated with pesticides g-HCH (lindane), DDT and p,p'-DMDT (methoxychlor) has been studied in batch tests. The treatments included different combinations of the following amendments: carbon source, reducing agent, zero-valent iron, surfactant and anaerobic biomass (methanogenic granular sludge or fermented sewage sludge). The tests seeded with both types of anaerobic biomass showed high removal of all pesticides. DDT was transformed into DDD, but accumulation of this metabolite was considerably lower than that resulting from stoichiometric reaction. Addition of a surfactant together with anaerobic biomass further enhanced the effectiveness of the process.
EN
Micellar and microemulsion systems in aqueous and nonaqueous phases may be used as media for biocatalytic processes, both using enzymes and whole cells. In the paper, the interactions between surfactants and enzymes, basic rules of micellar and microemulsion enzymatic catalysis and some interesting results both in aqueous and nonaqueous systems are presented.
EN
Pseudomonas aeruginosa and Pseudomonas putida, as Grampositive and Bacillus subtilis and Bacillus licheniformis as Gramnegative strains were used as biological agents in biodegradation process of model mixture of hydrocarbons alone. Furthermore in the presence of the synthetic emulsifiers of polyoxyethylenated alcohol and alkylglucoside derivatives, natural emulsifier of rhamnolipid derivatives and anionic surfactants were used.
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