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Protein microencapsulation ? methods and application

100%

Piasecka A., Goderska K.

Biotechnologia

2010

issue 1

34-45

Biomaterials extracted from natural environment may cause several serious problems due to their low stability. More efficient and safe procedures of their stabilization are still demanded to meet the industry requirements. Microencapsulation is one of the possible methods to prolong the action time of active compounds. This paper reports the techniques for preparing microcapsules and their applications with reference to extremely troublesome materials such as native proteins. Special attention is paid to current achievements in microencapsulation and perspectives for the next years, and advantages of this technique. The technology enables reactive or sensitive biomaterials such as enzymes to be turned into stable ingredients. With carefully controlled and fine-tuned release capabilities, microencapsulation is no longer only an added value technique, but the source of totally new systems with matchless properties.

Synthesis of recombinant proteins in the Pichia pastoris expression system

75%

Swiatek W.

Biotechnologia

2002

issue 4

180-209

During the past 15 years, the methylotrophic yeast Pichia pastoris has proven to be an excellent host for the production of both secreted and intracellular proteins. Numerous heterologous proteins have been produced at greater than gram per liter levels using alcohol oxidase promoter. The increasing popularity of this particular expression system can be attributed to several factors, most importantly: the simplicity of techniques for genetic manipulation, the ability to produce foreign proteins at high levels and the capability to perform many posttranslational modifications. The factors that drastically influence protein expression in this system include: copy number of the expression cassette, site and model of chromosomal integration of the heterologous gene, mRNA sequence and secondary structure, transcriptional and translational blocks, nature of secretion signal, endogenous proteases, host strain physiology, media and growth conditions. In this paper, I review how the system was developed, how it works and what can be done about it in the future.

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2 Biotechnologia

1 2010

1 2002

1 Goderska K.

1 Piasecka A.

1 Swiatek W.

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Protein microencapsulation ? methods and application

Synthesis of recombinant proteins in the Pichia pastoris expression system