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1

Artificial seeds for plant propagation

100%
Grajek W., Lassocinski W.
Biotechnologia
|
1993
|
issue 1
77-83
EN
Artificial seeds consisting of somatic embryos encapsulated with protective coating, have been proposed as a propagation system of plant propagation. Two types of artificial seeds have been developed, hydrated immobilized in hydrogels, and desiccated. The process of somatic embriogenesis runs in some steps. At first, the embryogenic tissue able to grow in suspension culture must be isolated. Subsequently, this tissue should be inducted by some auxins and kinetins introduced to the liquid medium to start the embryogenesis. The development and maturation of embryos carry out in special media. To scale up this process the buble column and air-lift bioreactors are used. The more frequently material to encapsulate the somatic embryos is alginate hydrogel. The main problem in production of good quality artificial seeds is low efficiency of embryos conversion to normal plant in soil.
2

Micropropagation of Leucojum aestivum L. in bioraector RITA?

100%
Ptak A., Gadek J.
Biotechnologia
|
2010
|
issue 2
165-171
EN
Embryogenic callus of Leucojum aestivum was transferred to solid and liquid media in bioreactor RITA? vessels. Callus growth on the liquid medium enriched with 2 and 5 M Picloram and 0.5 M BA was characterized by a higher multiplication index. The greatest number of somatic embryos was observed on the callus multiplied in bioreactor using 5-minute flushing with the medium. Globular embryos developed into torpedo-stage embryos and plantlets under the influence of NAA (0.5 M) and zeatin (5 M). Liquid medium promoted the development of L. aestivum somatic embryos and plantlets.
3

Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale

100%
Slusarkiewicz J. A., Ponitka A.
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|
vol. 38
|
issue 3
253-258
EN
Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (CHUANG et al. 1978) with increased 2,4 D to 2 mg L 1 and agarose 6 g L 1; 2 - Macro , micronutrients and vitamines like in MN6 (CHU, HILL 1988) + 2 mg L 1 2.4 D + 0.5 mg L 1 KIN + 5 mg L 1 FeEDTA + 90 g L 1 sucrose; 3 - Macro , micronutrients and vitamines like in MN6 (CHU, HILL, 1988) + 2 mg L 1 2.4 D + 0.5 mg L 1 KIN + 5 mg L 1 FeEDTA + 120 g L 1 sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64 1250, 12 486 and 6 212 somatic embryos and 8 86, 3 136 and 1 26 green plants were recorded, on media 1, 2 and 3, respectively.
4

Anther culture response in F1 hybrids of winter wheat (Triticum aestivum L.)

75%
Ponitka A., Slusarkiewicz-Jarzina A.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 3
253-260
EN
The effect of cold pretreatment of spikes on somatic embryo induction and ather culture response of 25 F1 winter hybrids was investigated.The efficiency of androgenic embryos was the highest when spikes were incubated at 4 C degree for 6-9 days.A total of 2242 (73%) green and 885 (27%) albino plants were obtained from 9900 cultured anthers.Anther culture response in wheat wsa found to be markedly affected by the genotype of donor plants.This percentage of green plants varied from 0 to 115.7%.The great majority of anther-derived regenerants were haploids (82.35%), while the remaining plants were spontaneous diploids (13,73%) and aneuploids (3.92%).
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