Oxycoccus quadripetalus callus induction and study on its capacity to regenerate shoots and/or roots were established.Woody Plant (WP) medium with 2iP (10 M) and NAA (1 M) was most suitable for callus tissue induction. The highest frequency of shoot regeneration was observed for WP medium supplemented with TDZ (0,2 M) and NAA (1 M). Multiplication of O. quadripetalus shoots from seedling shoot tips was also realized. Ca 3 (7 cm long) shoots with 11 nodes per shoot were differentiated from one explant.
The Oxalis triangularis transformed plants were regenerated from hairy roots induced by A. rhizogenes LBA 9402. In vitro shoot proliferation of the transformed roots-regenerated plants was 65% higher by than that of the non-transformed ones. In vivo the hairy root syndrome was observed for the transformed roots-regenerated plants. Anthocyanin content in the leaves of non-transformed plants was about 40% higher than in the transformed roots-regenerated ones. In the non-transformed plants, GSH (1,5 mM) enhanced anthocyanin production by 30% at the most, while in the transformed roots-regenerated plants the maximal increase in its production was found on the 2nd and 9th days after the treatment, it reached 230-320% and 750-600% of control, respectively.
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