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Structure and serological characterization of an N-[(R)-1-carboxyethyl]-L- -lysine-containing the O-chain of the lipopolysaccharide of Proteus mirabilis O13

100%

Swierzko A. A., Cedzynski M., Ziolkowski A., Senchenkova S. N., Perepelov A. V., Knierel Y. A., Kaca W.

Archivum Immunologiae et Therapiae Experimentalis

2001

vol. 49

issue 2

163-170

In this paper we present the structure and describe serological properties of the O-specific polysaccharide of Proteus mirabilis O13 lipopolysaccharide, which contains a unique component, an amide of D-galacturonic acid (D-GalA) with an unusual amino acid N-[(R)-1-carboxyethyl]-L-lysine (alaninolysine, AlaLys). Selective chemical degradations of either GalA or AlaLys resulted in the loss of the serological reactivity of the polysaccharide with anti-O serum against P. mirabilis O13. Neither synthetic stereoisomers of AlaLys nor the isolated amide of GalA with AlaLys inhibited the reaction of the O-antiserum with the homologous lipopolysaccharide. The O-antiserum did not cross-react with the lipopolysaccharide of Providencia alcalifaciens O23 containing an amide of D-glucuronic acid with AlaLys. These data showed that both uronic acid and amino acid components of the amide play an important role in manifesting the P. mirabilis O13-specificity, but the full specific epitope also includes also another OPS component(s). A cross-reactivity of anti-O13 serum with some other P. mirabilis strains was observed and attributed to a common heat-stable antigen(s) different from the lipopolysaccharide.

Immunochemical studies on the O-antigens of Proteus mirabilis O23 and Proteus vulgaris O23

88%

Rozalski A., Perepelov A. V., Bartodziejska B., Senchenkova S. N., Babicka D., Kaca W., Knirel Y. A.

Archivum Immunologiae et Therapiae Experimentalis

2003

vol. 51

issue 1

69-73

Analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy demonstrated that the O-specific polysaccharides of P. mirabilis PrK 42/57 and P. vulgaris PrK 43/57 are structurally similar to that of P. vulgaris PrK 44/57 and different from the polysaccharide of P. mirabilis PrK 41/57 studied earlier. The lipopolysaccharides of these strains were tested using enzyme immunosorbent assay, passive hemolysis and Western blot with O-antisera against P. mirabilis 42/57 and P. vulgaris 43/57 and 44/57, as well as with cross-absorbed O-antisera. The chemical and serological data revealed the basis for combining the four strains into Proteus serogroup O23 and division of this serogroup to three subgroups, one for P. vulgaris 43/57 and 44/57 and two others for P. mirabilis 41/57 and 42/57.

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2003

1 2001

2 Kaca W.

2 Perepelov A. V.

2 Senchenkova S. N.

1 Babicka D.

1 Bartodziejska B.

1 Cedzynski M.

1 Knierel Y. A.

1 Knirel Y. A.

1 Rozalski A.

1 Swierzko A. A.

1 Ziolkowski A.

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Structure and serological characterization of an N-[(R)-1-carboxyethyl]-L- -lysine-containing the O-chain of the lipopolysaccharide of Proteus mirabilis O13

Immunochemical studies on the O-antigens of Proteus mirabilis O23 and Proteus vulgaris O23