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1

The separation of biotechnology products in aqueous two-phase systems. Part II ? Applications

100%
Zielinska-Dawidziak M., Jankowski T., Zieli?ska-Dawidziak M.
Biotechnologia
|
2002
|
issue 2
102-112
EN
This paper is focused on actual and potential applications of aqueous two-phase systems in the separation of biotechnology products. Applications of bioconversions, purification of enzymes and other biomolecules in these systems, proposed schemes of separation, and advantages and disadvantages are presented. The experimental and industrial results indicate high efficiency of the separations of biological materials in aqueous two-phase systems, which may result in the development of new technologies.
2

The separation of biotechnology products in aqueous two-phase systems. Part I ? Theoretical background

80%
Zielinska-Dawidziak M., Jankowski T.
Biotechnologia
|
2002
|
issue 1
142-152
EN
The article presents the principles of liquid-liquid extraction in aqueous two-phase systems which offer a great potential for the recovery and purification of various biomolecules, cells and organelles from fermentation slurries. These systems consisting of a solution of two polymers (e.g. polyethylene glycol/dextran), polymer and salt (e.g. polyethylene glycol/phosphate) or a thermoseparating polymer (oxyethylene-oxypropylene copolymer) offer mild separation conditions due to the high concentration of water, which makes them suitable for biotechnology applications.
3

Separation of megabased-sized DNA molecules of Aspergillus niger using pulsed field gel electrophoresis.

80%
Coral G., Colak O., Unaldi M. N.
Folia Biologica
|
2002
|
vol. 50
|
issue 1-2
49-52
EN
In this study, the chromosomal DNAs were extracted from Aspergillus niger Z10 wild type strain and these DNAs were separated using the contour clamped homogeneous electric field gel electrophoresis (CHEF) system. This system is laboratory-made and is operated by a computer program. Total DNAs resolved into five distinct chromosomal bands. The size of the chromosomes was estimated as being between 3.3 Mb to 6.4 Mb.
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