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1

In vitro culture of Gentiana kurroo (Royle)

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Fiuk A., Rajkiewicz M., Rybczynski J. J.
Biotechnologia
|
2003
|
issue 3
267-274
EN
The aim of the presented paper is to show the present status of in vitro studies of Gentiana kurroo (Royle). Experimental material originated from seedlings, organs of regenerants, callus tissue, cell suspensions and isolated protoplasts. Two years of experiments proved very high morphological potential of plant material originated from various system of cultures. The mostly used media were those based on Murashige and Skoog medium, but for protoplast cultures, Kao and Michaluk medium appeared to be most useful. Numerous plant growth regulators with various concentrations and combinations controled plant regeneration of studied gentiana. Plant regeneration via somatic embriogenesis was observed in the majority of the studied culture systems.
2

Salt tolerance of Triticum monococcum L., T. dicoccum (Schrank) Schubl., T. durum Desf. and T. aestivum L. seedlings

100%
Prazak R.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 3
289-292
EN
Responses to salt stress of diploid wheat Triticum monococcum L., tetraploid wheats T. dicoccum (Schrank) Schubl. and T. durum Desf. cv. Grandur, and hexaploid wheats T. aestivum L. cvs. Begra and Gama were studied. Seeds were germinated on filter paper in Petri dishes, moistened in Hoagland medium containing 0 (control), 10, 20, 50, 100 and 200 mM NaCl. Index of tolerance to salt treatments was calculated on the basis of shoot length. Increase in salt concentration led to decrease in seed germination and inhibition of shoot and root elongation. Hexaploid and diploid wheats were more tolerant to salt stress than tetraploid wheats.
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