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1
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Surfactant-assisted polyol preparation of nickel powders with different morphologies

100%
Şişman İ., Tütünoğlu Ç., Aydın A.
Open Chemistry
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2008
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vol. 6
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issue 2
253-257
EN
Sub-micrometer nickel powders of controlled size and morphology were produced by a surfactant-assisted polyol method, using ethylene glycol (EG) as solvent and reductant in the presence of sodium dodecyl sulfate (SDS) surfactant and NaOH. The resultant Ni powders were characterized by XRD, SEM, EDS, and FTIR. Spherical, hexagonal, and triangular fcc Ni powders from 0.30 to 0.60 μm were obtained in the presence of SDS; irregular spherical fcc Ni powders were obtained in its absence. The concentrations of SDS, NaOH and Ni(CH3COO)2·4H2O greatly influence the product morphology and size. [...]
2
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Does the peroxidase-like activity of sodium dodecyl sulphate-modified cytochrome c increase after peroxynitrite or radiation treatment?.

100%
Gębicka L., Didik J.
|
2005
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vol. 52
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issue 2
551-555
EN
The peroxidase-like activity of cytochrome c is considerably increased by unfolding of the protein. The enhancement of the activity is due to the higher reaction rate of unfolded cytochrome c with hydrogen peroxide, which is the rate-determining step in the peroxidase cycle of cytochrome c (Gębicka, L., 2001, Res Chem Intermed 27, 717-23). In this study we checked whether combined action of two unfolding factors, SDS and peroxynitrite or radiation (hydroxyl radicals), increases the peroxidase-like activity of cytochrome c more than any single treatment alone. Peroxynitrite reacts with SDS-modified cytochrome c in the same way as with native cytochrome c, via intermediate radical products, •OH/•NO2, arising from peroxynitrite homolysis. We found that SDS-modified cytochrome c is much more sensitive to oxidative damage than the native protein. Partial unfolding of cytochrome c by SDS causes the peroxide substrate to have a better access to the heme center. On the other hand, the amino acids located in the vicinity of the active site and/or heme group become accessible for oxidizing radicals. The overall effect observed is that the peroxidase-like activity of SDS-modified cytochrome c decreases with an increase of the concentration of the oxidizing species (peroxynitrite or radiolytically generated hydroxyl radicals). The damage of SDS-modified cytochrome c caused by irradiation is much more significant than that observed after peroxynitrite treatment.
3
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A sample preparation method for micro-scale membrane proteome analysis

88%
Li J., Yu H., Duan Z., Wang X.
Sample Preparation
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2013
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vol. 1
28-37
EN
Detergents are widely used to improve the solubilization and extraction of hydrophobic membrane proteins in proteomics. Since most detergents are not compatible with subsequent steps of analysis, the removal of detergents from samples, especially those in micro-scale amounts, is a worthy topic of investigation. In this paper, we present a novel polyvinylidene difluoride (PVDF) membrane-mediated sample preparation method for micro-scale membrane proteome analysis, using a rat liver cell membrane-enriched fraction as model material. The proteins in the fraction were extracted in a 2 % sodium dodecyl sulfate (SDS) solution and the protein solution was applied on a piece of PVDF membrane followed by drying and repeated washing in order to remove SDS and other salts. Quantitative determination indicated that about 84% of the SDS in the sample was removed and protein loss was less than 10%. Four methods were used and compared for digesting the proteins adsorbed on PVDF membrane. Dimethyl formamide (DMF)-assisted digestion was the most effective with regard to the identification of membrane proteins, particularly the highly hydrophobic multi-transmembrane proteins. These results demonstrate that PVDF membrane-aided sample cleanup combined with DMF-assisted digestion has potential utility in the micro-scale membrane proteome analysis.
4
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Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

88%
Petruczynik A., Noster G., Waksmundzka-Hajnos M.
Current Issues in Pharmacy and Medical Sciences
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2015
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vol. 28
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issue 2
126-130
EN
In this work, the effects of sodium dodecyl sulfate (SDS) concentrations on retention, separation selectivity, peak shapes and systems efficiency were investigated. Herein, the retention data for 11 alkaloids were determined on an RP18 silica column with mobile phases containing methanol as organic modifier, with acetate buffer at pH 3.5, and, subsequently, with the addition of sodium dodecyl sulfate (SDS). The results of this study indicate that the retention of alkaloids decreases with the increase of SDS concentration in the mobile phase. The increase of SDS concentration, however, leads to the significantly improvement of peak symmetry and the increase of theoretical plate number in all cases. The best system efficiency for most of the investigated alkaloids was obtained in a mobile phase containing 0.1 M SDS, while most symmetrical peaks were obtained through the addition of 0.3 M of SDS to the mobile phase.
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