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1

A quantitative bacterial micro-assay for rapid detection of serum phenylalanine in dry blood-spots: Application in phenylketonuria screening

100%
Vallian S., Moeini H.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 1
79-83
EN
Phenylketonuria is an inherited metabolic disease, which is characterized by increased level of serum phenylalanine (Phe). The quantitative measurement of Phe in the serum is necessary to confirm the disease, and to distinguish phenylketonuria from other forms of hyperphenylalaninemia. In this study, we report a rapid and inexpensive micro-assay for simultaneous detection and quantitative measurement of serum Phe in dry blood-spots. Analysis of the standard curve showed a broad linear Phe range of 120?1800 mumol L?1. Application of this method in conjunction with the standard Guthrie bacterial inhibition assay and high-pressure liquid chromatography in analyzing 34 samples from phenylketonuria patients and control samples produced comparable results, with the regression equation of Y= 0.994X + 0.996. The advantage of this method over the Guthrie bacterial inhibition assay is its ability to measure the serum Phe quantitatively without false positive results. The method was successfully applied to dried blood-spots as well as serum and whole blood samples. The cost per sample is about 20?50 US cents, which is much less than those of high-pressure liquid chromatography and enzymatic commercial kits. The method can be automated, which is suitable for neonatal and mass phenylketonuria screening, especially in developing countries, where funding is a limiting factor.
2

Screening methods of industrial strain mutants

88%
Galas E., Kwapisz E., Polak J.
Biotechnologia
|
1995
|
issue 3
104-119
EN
The screening methods used for improvement of industrial strains are presented.Two general categories of methods have been distinguished: random screening and rational screening.Major techniques employed in rational screening are based on the selection of auxotropic mutants, reverants of non-producers, strain resistant to some factors like toxic analogues of metabolism intermediates, induction and catabolite regression or end-product inhibition, mutants with modified cell wall permeability and microorganisms tolerating somenutrients and precursors of secondary metabolites.
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