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The influence of salicylic and salicylhydroxsamic acid on in vitro potato plants growth was examined. Both solutions in higher contentrations (10-6, 10-7 mol dcm-3 SAL and 2 x 10-6, 2 x 10-7 mol dcm-3 SHAM) inhibited the growth of the shoots and the number of the leaves and roots. Lower concentrations (10-8 and 2x10-8 mol dcm-3), however, stimulated these processes. The inhibition and stimulation effects on cultivar 'Irga' and 'Irys' were observed. The intensity of tuberization was lower on the medium with the studied acids than in the control cultures. The level of linolenic acid was higher in plants with tuberizing potatoes. It suggests the inhibition of biosynthesis of the jasmonic acid (the stimulus of tuberization) by linolenic acid.
EN
Animals possess an inducible antibody immune system that acts as a defence against diseases.It is known that plants can also be active immunized against disease-causing pathogenes.This phenomenon is a result of the development of systemic acquired resistance (SAR).This article discussed recent studies on the role of salicilic acid (SA) in plants during the development of SAR.The understanding of the molecular and physiological background SAR may be applied in modern agrobiotechnology and pharmaceutical industry.
EN
Recognition of avirulent pathogens by plants activates defense system, cell death and the general broad-spectrum resistance called systemic acquired resistance - SAR. Several components involved in signaling resistance have recently been identified. Resistance gene mediated responses have been classified according to requirement of NDR1 or EDS1 gene. This classification correlates with R-gene structure. Salicylic acid plays central role in SAR. CPR and NPR1 genes function upstream and downstream of salicylic acid, respectively. Recent studies have demonstrated importance of the cell death in SAR. Novel defence signaling pathways that are independent on salicylic acid have been characterized.
EN
The effect of salicylic acid (SA) applied during the proliferation phase of cell suspension in vitro regeneration system of Medicago sativa L. on cell suspension growth, production of somatic embryos and their following development, germination and conversion were examined. SA is a potent inhibitor of the above processes. It is suggested that endogenous jasmonates are of some importance in regulation of somatic embryogenesis.
EN
Wheat germin, a glycoprotein mainly localized in the cell wall of monocots, dicots and pine (Pinus caribaea Morelet), is a specific marker of the onset of growth in germinating seeds. There are two genes gf-2.8 and gf-3.8 of germin, isolated from wheat (Triticum aestivum). Because of their structure and biochemistry, both of the germin's isoforms have been now assimilated to the oxalate-oxidase enzyme (E.C. 1.2.3.4). Several constructs of the gf-2.8 and gf-3.8 genes promoters deletion were fused with uidA gene and tested for the GUS activity in the transgenic tobacco plants. The aim of this work was to evaluate whether some environmental factors induce the activity of germin. Hormonal treatment (auxins 2,4-D, NAA, gibberellin GA3), salt treatment, salicylic acid, heavy metals (Mn, Fe, Co, Ni, Cu, Zn, Cd, Hg, As) and A1 induced high GUS activity in root apex, cotyledons and hypocotyls in transgenic seedlings and in leaves of the tobacco plants transformed with the entire and one partially deleted construct of the gf-2.8 gene. Neither cold nor heat shock, enhanced significant GUS activity. The gf-3.8 - GUS constructs of germin did not give a positive response to any factors mentioned above. Thus, only the gf-2.8 protein seems to be regulated by some factors. The gf-2.8 oxalate-oxidase activity could be then involved in general stress-induced signalling in higher plants
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