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  1. 1 Biotechnologia

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  1. 1 2011

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  1. 1 Cydzik-Kwiatkowska A.

  2. 1 Wnuk M.

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Optimization of activated sludge storage before RNA isolation

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Cydzik-Kwiatkowska A., Wnuk M.
Biotechnologia
|
2011
|
issue 1
101-105
EN
Proper sample storage before RNA isolation is crucial for a reliable assessment of microbial activity in activated sludge. In our studies, different amounts of activated sludge expressed in mg of total suspended solids (TSS) were fixed with a constant volume of RNA later RNA Stabilization Reagent (QIAGEN, Germantown, MD, USA) and stored for 3 or 6 months. The results obtained point out that for the best RNA recovery, 1 mg of activated sludge TSS should be fixed with 33 ?l of the RNAlater. It was also observed that a longer storage period (6 vs. 3 months) of the activated sludge in RNA later lowered RNA recovery by, on average, 25 ? 15% although bacterial diversity in all samples remained unaltered.
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