Changes in expression of estrogen receptor alpha (ER) mRNA were studied in special reference to follicular growth of the ovarian follicles in laying quail. Levels of mRNA were determined by RT-PCR in the ovarian stroma, each class of the ovarian follicles, oviductal parts and in the liver. Low levels of ER mRNA were detected in stroma, the small white follicles, large white follicles and small yellow follicles and in the theca layer of the three largest preovulatoy follicles. Although the level in the granulosa layer of the F3 was also low, the level significantly increased in F2 and F1. Relatively higher levels were found in the liver and oviduct, and that in the magnum was the highest among all tissues examined. The level in the F1 granulosa layer was comparable to that in the liver which actively synthesises egg yolk proteins for the sake of estrogen and ER. The results of the present study demonstrate that (1) ER mRNA is present in each compartment of the reproductive tissue in quail, (2) the marked expression of ER mRNA in the granulosa layer of the largest follicle may indicate the involvement of estrogens in the biosynthesis of inhibin/activin, progesterone and yolk perivitelline layer protein, (3) very high expression of ER in the oviductal tissues may be related to the role of estrogens in cell proliferation and protein synthesis in the oviduct.
Karyotypes of four species of Pachypsyllinae were reinvestigated (Pachypsylla celtidismamma, P. celtidisvesiculum, P. venusta, Pachypsylla sp. 2, and Tetragonocephala flava) in order to interpret contradiction in the literature data. The morphology of testicular follicles for three species was described for the first time.
Karyotypes (number and size of chromosomes, sex -determining system) and testis structure (number of testicular follicles) of 37 species belonging to 24 genera of the little studied fulgoroid families Cixiidae, Meenoplidae, Derbidae, Achilidae, Nogodinidae, Tropiduchidae and Flatidae are presented. For Meenoplidae, Nogodinidae and Tropiduchidae data on the testis structure have been obtained for the first time. The data available for Fulgoroidea as a whole are reviewed.
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