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EN
Ten Polish red clover cultivars were tested in vitro for their capability to produce callus and somatic embryos. A three-step tissue culture protocol (callus induction, embryo induction and plant development) based on Gamborg's B5 basal salts, NAA and 2,4-D as auxins, and kinetin and adenine as cytokinins was utilized. Explants source were petiole and hypocotyl sections. All clover varieties under study have showed high efficiency of callus production, and low ability for plant regeneration from the callus. The highest percent of genotypes which developed embryos was observed on variety Parka (3,6). Single embryogenic genotypes were selected from nearly all varieties. Hypocotyls showed the greatest ability for callus regeneration comparing to leaf petioles.
EN
Anthocyanin spot on the leaves of red clover is inherited indepently of light spot and uniform leaf colour.The gene determining anthocyanin spot is probably localized on the other chromosomes than the gene determining light spot on the leaves.A short flower tube is also determined by a single recessive gene of a pleiotropic action.It stimultaneously causes reduction of pistil style, thickening of the endithecium layer in anthers - entails their indehiscence, pollen grain-agglutination and often deformation, no division of part of pollen grains into a vegetative and generative cells as well as germination of a single grains in pollen sacks.The changes in the structure of endothecium and pollen grains cause almost complete male and female infertility of plants with short flower tubes.
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